AMT12 Heterotrophic nanoflagellates sampled by CTD bottle
Originator's Protocol for Data Acquisition and Analysis
Seawater samples were collected from the CTD Niskin bottles at the pre-dawn cast from up to 5 light depths. Water samples were fixed with Lugol's solution and formalin directly from the CTD rossette bottles. Slides were prepared within 24 hours for analysis after the cruise. The slides were examined using epifluorescent microscopy with an inverted microscope at x200 magnification.
References Cited
Instrumentation Description
Not applicable for this data set.
BODC Data Processing Procedures
Data were submitted via email in an Excel spreadsheet archived under BODC's accession number PML100120. Sample metadata (Cruise, CTD cast, date, latitude, longitude, and depth) were checked against information held in the database. There were four discrepancies. The date, position and depths sampled for CTD casts 2 and 13 in the originator's file matched CTD casts 3 and 12 respectively in the database and so the data were loaded against this cast. There were two depth discrepancies one for cast 40 and one for cast 51, where the data originator had provided a sample from 65m and 140m respectively at which no bottles were fired. There was a bottle fired at 56m on cast 40 and 122m on cast 51 and the data were loaded against these bottle samples.
Parameter codes defined in BODC parameter dictionary were assigned to the variables. The abundance data were provided as cells per millilitre and the biomass data were provided as micrograms per litre, which were consistent with the units for the relevant parameters in the BODC database.
The data were reformatted and loaded in BODC's samples database under Oracle Relational Database Management System. Data were marked up with BODC parameter codes and loaded into the database. Individual samples were matched by cast and depth.
A parameter mapping table is provided below;
Originator's Parameter | Units | Description | BODC Parameter Code | Units | Comments |
---|---|---|---|---|---|
Abundance | cells ml -1 | Abundance of flagellate [Size: 2-20µm Subgroup: heterotrophic] per unit volume of the water body by DAPI/proflavine staining and epifluorescence microscopy | P400E00B | cells ml -1 | n/a |
Biomass | µg C l -1 | Carbon biomass of flagellate [Size: 2-20µm Subgroup: heterotrophic] per unit volume of the water body by DAPI/proflavine staining and epifluorescence microscopy and abundance to carbon conversion by unspecified algorithm | C400E00B | mg C m -3 | No unit conversion necessary as units are equivalent |
Data Quality Report
BODC were not advised of specific quality checks carried out by the data originator.
Problem Report
Not relevant to this data set.