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BODC Cruise Metadata Report for FS Poseidon (06PO) cruise PO311

Introduction

This report collates information from the BODC cruise inventory and oceanographic databases. It presents

Cruise Inventory Information

Long name FS Poseidon (06PO) cruise PO311
Short name PO311
Objectives and Narrative Between the 24th April and 26th May 2004 Plymouth Marine Laboratory in collaboration with scientists from NIWA, New Zealand, Laboratoire Arago, France and University of East Anglia conducted a two-ship exercise using RRS Charles Darwin (see Charles Darwin cruise CD156) and RV Poseidon to test the hypothesis that:
The supply of, and the interaction between iron and phosphorous control biological activity and fluxes in the subtropical North Atlantic.

An experimental area in international waters to the west of the Canary Islands was selected following an intense period of vertical and horizontal mapping by the Charles Darwin. Using SF6 as a tracer for amended waters, two separate experiments were performed:
The first (5th - 15th May) involved the addition of 20 tonnes of anhydrous monosodium phosphate at 10 m depth over an area of approximately 25 km2, centered at 27.8degN 23.3deg;
The second experiment was conducted following a mid-cruise return by the Poseidon to Tenerife between 16th May and 22nd May, at 27.5degN 22.5degW. 5 tonnes of acidified iron sulphate were added over the first 12 hours, then following a brief recovery period, 20 tonnes of phosphate added over the top of the iron.

Measurements of nutrient chemistry, gas exchange and biological activity were monitored prior to and after deployment (IN stations) of the fertilised patches relative to several (OUT) control stations.
Ship FS Poseidon
Departure Port Santa Cruz de Tenerife, Spain
Departure Date 2004-04-29
Arrival Port Santa Cruz de Tenerife, Spain
Arrival Date 2004-05-24
Principal Scientist(s) Andrew Rees (Plymouth Marine Laboratory)
Responsible Organisation Scottish Marine Biological Association
Associated Project(s) FEEP
IHB Sea Areas Visited North East Atlantic Ocean (limit 40W)
Marsden Squares Visited 75
Work Area Description subtropical North-East Atlantic to the west of the Canary Islands
Work Area Bounding Polygon 20N,30W; 30N,30W; 30N,20W; 20N,20W; 20N,30W
Southern Limit 20N
Northern Limit 30N
Western Limit 30W
Eastern Limit 20W

Cruise Inventory Datasets

Physical oceanography  
CTD stations Quantity: number of profiles = 127
Description: 12 bottle rosette, Seabird 911 CTD unit, Dr Haardt Fluorimeter Beckmann/YSI Oxygen probe.  
Geochemical tracers (eg freons) Quantity: number of measurements = 210
Description: Onboard GC analysis of SF6 concentration following deliberate release to surface waters.  
Chemical oceanography  
Ammonia Quantity: number of samples = 330
Description: Water for ammonium analysis on samples collected from CTD bottles using manual method and analysed onboard using a flurometer.  
Ammonia Quantity: number of samples = 360
Description: Water samples from 1% and 55% sPAR underwent deck incubations with 15NH4 Samples were then filtered, derivitised and the NH4+ collected by solid phase extraction.  
Ammonia Quantity: number of samples = 144
Description: Filtered seawater for 15 N ammonium regeneration onto C18 GCMS columns from experimental bottles (size fractionated ammonium regeneration experiments). Analysis by GCMS in laboratory.  
Ammonia Quantity: number of samples = 96
Description: Filtered seawater for 15 N ammonium uptake in experimental bottles (size fractionated ammonium regeneration experiments). Mass Spec analysis in laboratory.  
Carbon dioxide Quantity: number of samples = 55
Description: Water for coulometric determination of dissolved inorganic carbon  
Isotopes Quantity: number of samples = 96
Description: Filtered seawater for 15 N ammonium uptake in experimental bottles (size fractionated ammonium regeneration experiments). Mass Spec analysis in laboratory.  
Isotopes Quantity: number of samples = 144
Description: Filtered seawater for 15 N ammonium regeneration onto C18 GCMS columns from experimental bottles (size fractionated ammonium regeneration experiments). Analysis by GCMS in laboratory.  
Isotopes Quantity: number of samples = 160
Description: Filtered seawater for 15N natural abundance measurements. Mass Spec analysis in laboratory  
Isotopes Quantity: number of samples = 200
Description: 15N-N2 nitrogen fixation incubations. These were filtered and the filters retained Mass spec analysis at a later date.  
Nitrate Quantity: number of samples = 240
Description: Water samples from 1% and 55% sPAR underwent deck incubations with 15NO2. Samples were then filtered, treated with a cadmium column for NO3 reduction to NO2, derivitised and the NO2- collected by solid phase extraction.  
Nitrate Quantity: number of samples = 350
Description: Water for nutrient analysis on samples collected from CTD bottles using Technicon AA2 AutoAnalyser  
Nitrate Quantity: number of samples = 100
Description: Water for nutrient analysis on samples collected during experimental procedures using Technicon AA2 AutoAnalyser  
Nitrite Quantity: number of samples = 350
Description: Water for nutrient analysis on samples collected from CTD bottles using Technicon AA2 AutoAnalyser  
Nitrite Quantity: number of samples = 100
Description: Water for nutrient analysis on samples collected during experimental procedures using Technicon AA2 AutoAnalyser  
Nitrite Quantity: number of samples = 480
Description: Water samples from 1% and 55% sPAR underwent deck incubations with 15N02. Samples were then filtered, derivitised and the NO3- collected by solid phase extraction.  
Other chemical oceanographic measurements Quantity: number of samples = 6
Description: Water samples filtered through 0.2 um filters and incubated for 10 hr for determination of photochemical consumption of oxygen.  
Oxygen Quantity: number of samples = 135
Description: Water for winkler dissolved oxygen concentration for calibration of the CTDs SBE oxygen sensors.  
Phosphate Quantity: number of samples = 350
Description: Water for nutrient analysis on samples collected from CTD bottles using Technicon AA2 AutoAnalyser  
Phosphate Quantity: number of samples = 100
Description: Water for nutrient analysis on samples collected during experimental procedures using Technicon AA2 AutoAnalyser  
Radioactivity Quantity: number of samples = 250
Description: Microbial phosphate uptake measurements based on radio-isotope (33P) labelling experiments (dawn to dusk) using on-deck incubators & cubitanor experiments. Includes size-fractionation at two depths.  
Radioactivity Quantity: number of samples = 250
Description: Primary production measurements based on radio-isotope (14C) phytoplankton labelling experiments (dawn to dusk) using on-deck incubators & cubitanor experiments. Includes size-fractionation at two depths.  
Silicate Quantity: number of samples = 100
Description: Water for nutrient analysis on samples collected during experimental procedures using Technicon AA2 AutoAnalyser  
Silicate Quantity: number of samples = 350
Description: Water for nutrient analysis on samples collected from CTD bottles using Technicon AA2 AutoAnalyser  
Biology and fisheries  
Pelagic bacteria/micro-organisms Quantity: number of samples = 200
Description: 15N-N2 nitrogen fixation incubations. These were filtered and the filters retained Mass spec analysis at a later date.  
Pelagic bacteria/micro-organisms Quantity: number of samples = 160
Description: Filtered seawater for 15N natural abundance measurements. Mass Spec analysis in laboratory  
Pelagic bacteria/micro-organisms Quantity: number of samples = 144
Description: Water for picoplankton abundance estimates from analyses of fixed samples (paraformaldehyde 1%) from experimental bottles (microzooplankton dilution experiments and size fractionated ammonium regeneration experiments). Analysis by flow cytometry in laboratory.  
Pelagic bacteria/micro-organisms Quantity: number of samples = 250
Description: Microbial phosphate uptake measurements based on radio-isotope (33P) labelling experiments (dawn to dusk) using on-deck incubators & cubitanor experiments. Includes size-fractionation at two depths.  
Pelagic bacteria/micro-organisms Quantity: number of stations = 250
Description: Heterotrophic bacterial production measurements from 6 depths using radiolabelled tracers (including cubitanor deck incubations)  
Pelagic bacteria/micro-organisms Quantity: number of measurements = 150
Description: Acetylene for a reduction experiment using FID-GC to determine nitrogen fixation in surface waters.  
Pelagic bacteria/micro-organisms Quantity: number of samples = 220
Description: 2 ml volume for picoplankton abundance estimates from analyses of fixed samples (paraformaldehyde 1%) from the CTD casts. Analysis by flow cytometry in laboratory.  
Phytoplankton Quantity: number of samples = 320
Description: Water samples fixed in Lugol's iodine 2% from the CTD casts for the later enumeration of phytoplankton and microzooplankton.  
Phytoplankton Quantity: number of samples = 70
Description: Video observations of successful hauls  
Phytoplankton Quantity: number of samples = 220
Description: Filtration of 9 litres seawater from 5 depths in surface 40 m onto 0.2ul sterivex filters for DNA extraction for analysis of nifH and bacterial diversity. Stored at -20degC for lab analysis.  
Phytoplankton pigs (eg chlorophyll, fluorescence) Quantity: number of samples = 108
Description: Chlorophyll-a samples collected from experimental bottles (microzooplankton dilution experiments and size fractionated ammonium regeneration experiments) and frozen for later analysis.  
Phytoplankton pigs (eg chlorophyll, fluorescence) Quantity: number of samples = 40
Description: Periodic chlorophyll-a samples to support various deck incubation experiments. Pigment extracted in 90% acetone  
Primary productivity Quantity: number of samples = 200
Description: 15N-N2 nitrogen fixation incubations. These were filtered and the filters retained Mass spec analysis at a later date.  
Primary productivity Quantity: number of samples = 160
Description: Filtered seawater for 15N natural abundance measurements. Mass Spec analysis in laboratory  
Primary productivity Quantity: number of measurements = 150
Description: Acetylene for a reduction experiment using FID-GC to determine nitrogen fixation in surface waters.  
Primary productivity Quantity: number of samples = 250
Description: Water samples for gross primary production, community respiration and net community production rate measuremens after 24 h deck incubations.  
Primary productivity Quantity: number of samples = 250
Description: Primary production measurements based on radio-isotope (14C) phytoplankton labelling experiments (dawn to dusk) using on-deck incubators & cubitanor experiments. Includes size-fractionation at two depths.  
Primary productivity Quantity: number of samples = 250
Description: Microbial phosphate uptake measurements based on radio-isotope (33P) labelling experiments (dawn to dusk) using on-deck incubators & cubitanor experiments. Includes size-fractionation at two depths.  
Zooplankton Quantity: number of samples = 108
Description: Water samples fixed in Lugol's iodine 2% from experimental bottles (microzooplankton dilution experiments and size fractionated ammonium regeneration experiments) for the later enumeration of microzooplankton.  
Zooplankton Quantity: number of samples = 72
Description: Epifluorescence staining of filters from filtration of seawater samples collected from CTD casts for later enumeration of flagellates by microscopy  
Zooplankton Quantity: number of samples = 320
Description: Water samples fixed in Lugol's iodine 2% from the CTD casts for the later enumeration of phytoplankton and microzooplankton.  
Zooplankton Quantity: number of hauls = 17
Description: 17 grazing experiments on Pleurommama sp. were set up on board to determine its diet.  
Zooplankton Quantity: number of hauls = 32
Description: Size fractionated zooplankton samples were frozen for biomass, enzyme activity and stable isotopes analysis.  
Zooplankton Quantity: number of hauls = 44
Description: Zooplankton was frozen for gut contents analysis by fluorescence at 44 stations - 10 gut evacuation rate experiments were conducted in parallel.  
Zooplankton Quantity: number of hauls = 44
Description: Zooplankton for biomass and community description were fixed in buffered formaldehyde 4%. Samples collected from 200 to 0 m with a 200 um mesh net on 44 stations  
Zooplankton Quantity: number of samples = 36
Description: Epifluorescence staining of filters from filtration of seawater samples from experimental bottles (microzooplankton dilution experiments and size fractionated ammonium regeneration experiments) for later enumeration of flagellates by microscopy.  

Cruise Inventory Mooring/Buoy Operations

No information on datasets is currently available

Summary of BODC Data Holdings for the Cruise

National Oceanographic Database

Summary

# Series Instrument Description Parameters
93 Bottle station Metadata parameters
Nitrate concentration parameters in the water column
Nitrite concentration parameters in the water column
Phosphate concentration parameters in the water column
Quality control flags
Reference numbers
Silicate concentration parameters in the water column
Vertical spatial coordinates

Note: Parameter terms are taken from the BODC Parameter Discovery Vocabulary XML (SKOS)

Full List

BODC Reference Instrument Description Date/Time Location  
1266441 Bottle station 2004-05-03 03:30Z 27° 47' N, 23° 20' W Click for more
1266453 Bottle station 2004-05-03 04:03Z 27° 47' N, 23° 20' W Click for more
1266465 Bottle station 2004-05-03 04:30Z 27° 47' N, 23° 20' W Click for more
1266477 Bottle station 2004-05-03 05:03Z 27° 47' N, 23° 20' W Click for more
1266489 Bottle station 2004-05-03 11:57Z 27° 48' N, 23° 19' W Click for more
1266490 Bottle station 2004-05-04 03:11Z 27° 48' N, 23° 19' W Click for more
1266508 Bottle station 2004-05-04 03:45Z 27° 47' N, 23° 19' W Click for more
1266521 Bottle station 2004-05-04 04:14Z 27° 47' N, 23° 19' W Click for more
1266533 Bottle station 2004-05-04 04:50Z 27° 48' N, 23° 19' W Click for more
1266545 Bottle station 2004-05-04 12:42Z 27° 48' N, 23° 19' W Click for more
1266557 Bottle station 2004-05-05 04:25Z 27° 48' N, 23° 16' W Click for more
1266569 Bottle station 2004-05-05 04:57Z 27° 47' N, 23° 16' W Click for more
1266570 Bottle station 2004-05-05 05:36Z 27° 46' N, 23° 17' W Click for more
1266582 Bottle station 2004-05-05 06:05Z 27° 46' N, 23° 16' W Click for more
1266594 Bottle station 2004-05-05 11:48Z 27° 46' N, 23° 18' W Click for more
1266601 Bottle station 2004-05-05 13:15Z 27° 46' N, 23° 18' W Click for more
1266613 Bottle station 2004-05-06 03:02Z 27° 46' N, 23° 16' W Click for more
1266625 Bottle station 2004-05-06 03:31Z 27° 46' N, 23° 16' W Click for more
1266637 Bottle station 2004-05-06 04:01Z 27° 46' N, 23° 16' W Click for more
1266649 Bottle station 2004-05-06 04:45Z 27° 45' N, 23° 15' W Click for more
1266650 Bottle station 2004-05-06 09:48Z 27° 45' N, 23° 16' W Click for more
1266662 Bottle station 2004-05-06 14:34Z 27° 44' N, 23° 23' W Click for more
1266674 Bottle station 2004-05-06 15:18Z 27° 44' N, 23° 23' W Click for more
1266686 Bottle station 2004-05-07 03:17Z 27° 45' N, 23° 23' W Click for more
1266698 Bottle station 2004-05-07 03:47Z 27° 44' N, 23° 22' W Click for more
1266705 Bottle station 2004-05-07 04:14Z 27° 44' N, 23° 22' W Click for more
1266717 Bottle station 2004-05-07 04:43Z 27° 43' N, 23° 22' W Click for more
1266729 Bottle station 2004-05-07 09:48Z 27° 44' N, 23° 23' W Click for more
1266730 Bottle station 2004-05-07 13:50Z 27° 44' N, 23° 13' W Click for more
1266742 Bottle station 2004-05-07 14:40Z 27° 44' N, 23° 13' W Click for more
1266754 Bottle station 2004-05-08 03:09Z 27° 42' N, 23° 15' W Click for more
1266766 Bottle station 2004-05-08 03:43Z 27° 42' N, 23° 15' W Click for more
1266778 Bottle station 2004-05-08 04:11Z 27° 42' N, 23° 15' W Click for more
1266791 Bottle station 2004-05-08 04:49Z 27° 42' N, 23° 15' W Click for more
1266809 Bottle station 2004-05-08 09:38Z 27° 40' N, 23° 13' W Click for more
1266810 Bottle station 2004-05-08 13:47Z 27° 30' N, 23° 13' W Click for more
1266822 Bottle station 2004-05-09 03:09Z 27° 30' N, 23° 12' W Click for more
1266834 Bottle station 2004-05-09 03:41Z 27° 30' N, 23° 12' W Click for more
1266846 Bottle station 2004-05-09 04:14Z 27° 30' N, 23° 11' W Click for more
1266858 Bottle station 2004-05-09 04:43Z 27° 30' N, 23° 10' W Click for more
1266871 Bottle station 2004-05-09 09:21Z 27° 28' N, 23° 12' W Click for more
1266883 Bottle station 2004-05-09 13:12Z 27° 41' N, 23° 12' W Click for more
1266895 Bottle station 2004-05-10 03:15Z 27° 39' N, 23° 15' W Click for more
1266902 Bottle station 2004-05-10 03:49Z 27° 39' N, 23° 13' W Click for more
1266914 Bottle station 2004-05-10 04:17Z 27° 39' N, 23° 13' W Click for more
1266926 Bottle station 2004-05-10 04:46Z 27° 39' N, 23° 13' W Click for more
1266938 Bottle station 2004-05-10 13:28Z 27° 40' N, 22° 56' W Click for more
1266951 Bottle station 2004-05-15 03:07Z 27° 37' N, 23° 16' W Click for more
1266963 Bottle station 2004-05-15 03:36Z 27° 36' N, 23° 16' W Click for more
1266975 Bottle station 2004-05-15 03:59Z 27° 36' N, 23° 16' W Click for more
1266987 Bottle station 2004-05-15 04:27Z 27° 36' N, 23° 16' W Click for more
1266999 Bottle station 2004-05-15 10:01Z 27° 36' N, 23° 16' W Click for more
1267002 Bottle station 2004-05-15 13:55Z 27° 36' N, 22° 49' W Click for more
1267014 Bottle station 2004-05-16 03:06Z 27° 31' N, 22° 34' W Click for more
1267026 Bottle station 2004-05-16 03:35Z 27° 31' N, 22° 33' W Click for more
1267038 Bottle station 2004-05-16 03:58Z 27° 31' N, 22° 33' W Click for more
1267051 Bottle station 2004-05-16 04:24Z 27° 31' N, 22° 33' W Click for more
1267063 Bottle station 2004-05-16 15:18Z 27° 33' N, 22° 32' W Click for more
1267075 Bottle station 2004-05-17 03:05Z 27° 33' N, 22° 29' W Click for more
1267087 Bottle station 2004-05-17 03:36Z 27° 33' N, 22° 28' W Click for more
1267099 Bottle station 2004-05-17 04:01Z 27° 33' N, 22° 28' W Click for more
1267106 Bottle station 2004-05-17 04:30Z 27° 33' N, 22° 28' W Click for more
1267118 Bottle station 2004-05-17 09:15Z 27° 37' N, 22° 28' W Click for more
1267131 Bottle station 2004-05-17 13:09Z 27° 33' N, 22° 27' W Click for more
1267143 Bottle station 2004-05-18 03:13Z 27° 35' N, 22° 23' W Click for more
1267155 Bottle station 2004-05-18 03:45Z 27° 34' N, 22° 22' W Click for more
1267167 Bottle station 2004-05-18 04:07Z 27° 34' N, 22° 22' W Click for more
1267179 Bottle station 2004-05-18 04:41Z 27° 34' N, 22° 22' W Click for more
1267180 Bottle station 2004-05-18 09:26Z 27° 34' N, 22° 22' W Click for more
1267192 Bottle station 2004-05-18 13:10Z 27° 33' N, 22° 31' W Click for more
1267211 Bottle station 2004-05-19 03:17Z 27° 34' N, 22° 32' W Click for more
1267223 Bottle station 2004-05-19 03:46Z 27° 34' N, 22° 33' W Click for more
1267235 Bottle station 2004-05-19 04:09Z 27° 34' N, 22° 32' W Click for more
1267247 Bottle station 2004-05-19 04:33Z 27° 34' N, 22° 32' W Click for more
1267259 Bottle station 2004-05-19 09:10Z 27° 34' N, 22° 33' W Click for more
1267260 Bottle station 2004-05-19 13:30Z 27° 40' N, 22° 16' W Click for more
1267272 Bottle station 2004-05-20 03:49Z 27° 39' N, 22° 16' W Click for more
1267284 Bottle station 2004-05-20 04:17Z 27° 39' N, 22° 16' W Click for more
1267296 Bottle station 2004-05-20 04:40Z 27° 39' N, 22° 16' W Click for more
1267303 Bottle station 2004-05-20 05:03Z 27° 39' N, 22° 16' W Click for more
1267315 Bottle station 2004-05-20 09:10Z 27° 39' N, 22° 16' W Click for more
1267327 Bottle station 2004-05-20 09:47Z 27° 39' N, 22° 16' W Click for more
1267339 Bottle station 2004-05-20 13:13Z 27° 37' N, 22° 21' W Click for more
1267340 Bottle station 2004-05-21 03:10Z 27° 38' N, 22° 22' W Click for more
1267352 Bottle station 2004-05-21 03:38Z 27° 38' N, 22° 22' W Click for more
1267364 Bottle station 2004-05-21 04:00Z 27° 39' N, 22° 22' W Click for more
1267376 Bottle station 2004-05-21 04:22Z 27° 39' N, 22° 22' W Click for more
1267388 Bottle station 2004-05-21 09:08Z 27° 39' N, 22° 23' W Click for more
1267407 Bottle station 2004-05-21 11:35Z 27° 40' N, 22° 12' W Click for more
1267419 Bottle station 2004-05-22 03:23Z 27° 41' N, 22° 6' W Click for more
1267420 Bottle station 2004-05-22 03:54Z 27° 41' N, 22° 6' W Click for more
1267432 Bottle station 2004-05-22 04:12Z 27° 41' N, 22° 6' W Click for more
1267444 Bottle station 2004-05-22 04:35Z 27° 41' N, 22° 6' W Click for more

Project Database

# Events Gear Type Parameters Held
127 CTD frame plus rosette sampler
Particulate total and organic nitrogen concentrations in the water column
Density of the water column
Electrical conductivity of the water column
Silicate concentration parameters in the water column
Raw fluorometer output
Salinity of the water column
Nitrate concentration parameters in the water column
Temperature of the water column
Nitrite concentration parameters in the water column
Phosphate concentration parameters in the water column
New production in water bodies
Dissolved oxygen parameters in the water column
8 Atmospheric air sampling pump Inorganic phosphorus species concentrations in the atmosphere
Metal concentrations in the atmosphere
Concentration of inorganic halogens in the atmosphere
Concentration of nitrogen species in the atmosphere
Concentrations of carboxylic acids and carboxylates in the atmosphere
Sulphur species concentrations in the atmosphere

Note: Parameter terms are taken from the BODC Parameter Discovery Vocabulary XML (SKOS)

References

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