AMT15 Pico-plankton, nano-plankton and bacteria abundance from AFC analysis of samples collected from CTD bottle samples
Originator's Protocol for Data Acquisition and Analysis
This data originates from analyses on samples collected from CTD casts during the cruise. Samples were taken from the pre-dawn CTD cast (~0200 - 0400h local time) and from the late morning 'optics' cast (1100h local time).
Seawater samples were collected in HCl washed 50ml polypropylene tubes from each depth on 3am and 11am casts. Subsamples were fixed with paraformaldehyde (1% final concentration) and frozen for flow cytometric analysis of autotrophic and heterotrophic picoplankton abundance post-cruise.
References Cited
Instrumentation Description
Becton Dickinson FACSort instrument
BODC Data Processing Procedures
Data were submitted via email in an Excel spreadsheet archived under BODC's accession number SOC060486. Sample metadata (CTD number, latitude/longitude and depth) were checked against information held in the database. Individual samples were matched through rosette sampling bottle and depth. There was one discrepancy between the metadata provided and that held in the database. Abundance data were provided for heterotrophic bacteria sampled from CTD cast 4 (CTD004), however the rosette bottles were reported as failing to fire for this cast. The data from this cast has not been loaded to the database.
The data were provided in cell abundance per millilitre. These units were consistent with the BODC parameter code units and no conversions were necessary.
The data were reformatted and loaded in BODC's samples database under Oracle Relational Database Management System. Data were marked up with BODC parameter codes and loaded into the database.
A parameter mapping table is provided below;
| Originator's Parameter | Units | Description | BODC Parameter Code | Units | Comments |
|---|---|---|---|---|---|
| Synechococcus spp. | cell abundance ml -1 | Abundance of Synechococcus spp. (ITIS: 773: WoRMS 160572) per unit volume of the water body by automated flow cytometry | P700A90Z | cell abundance ml -1 | n/a |
| Prochlorococcus spp. | cell abundance ml -1 | Abundance of Prochlorococcus spp. (ITIS: 610076: WoRMS 345515) per unit volume of the water body by automated flow cytometry | P701A90Z | cell abundance ml -1 | n/a |
| Heterotrophic bacteria | cell abundance ml -1 | Abundance of Bacteria (ITIS: 202421: WoRMS 6) [Subgroup: heterotrophic] per unit volume of the water body by automated flow cytometry and subtraction of Synechococcus+Prochlorococcus from total bacteria | HBCCAFTX | cell abundance ml -1 | n/a |
Data Quality Report
BODC were not advised of specific quality checks carried out by the data originators. There were no standout values in the sample data provided to BODC.
Problem Report
Not relevant to this data set.
