AMT19 (JC039) pigment composition by HPLC analysis from surface underway samples
Data Acquisition and Analysis
Fresh seawater samples from the ship's non-toxic supply were collected during the cruise. 2-4 L of seawater were filtered through Whatman GF/F filters and the filters stored at -80°C. HPLC analysis was carried out after Van Heukelem and Thomas (2001).
References Cited
Van Heukelem, L. and Thomas, C. S. (2001). Computer-assisted high performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments, Journal of Chromatography A, 910, 31-49.
Instrumentation Description
Section not relevant.
BODC Data Processing Procedures
Data were submitted to BODC in the SeaBASS text format and saved to the archive with reference PML110038. The file was provided to BODC with date, time (in GMT), latitude, longitude and sample depth as metadata.
Sample metadata were checked against information held in the cruise report - there was one discrepancy where all sample depths provided were 5m except one which was provided as 0m, this was confirmed as a typo with the data originator. The metadata were loaded to the database.
Parameter codes defined in BODC parameter dictionary were assigned to the variables as shown in the table below. The data were provided in mg m -3 units and these were converted to ng l -1 for all parameters but chlorophyll-a. Three parameters were not loaded to the database (Tot chl-a, Tot chl-b and Tot chl-c) as these were derived by aggregating other parameters in the dataset. These parameters can be derived from the following parameters:
Tot_Chl_a = Chl_a + DV_Chl_a + Chlide_a
Tot_Chl_b = Chl_b + DV_Chl_b
Tot_Chl_c = Chl_c1c2 + Chl_c3
There were 18 sample events with data from replicate samples provided and these data were averaged and the mean and standard deviation values loaded to the database for each sampling event.
Data loaded into BODC's database according to protocols.
| Originator's Parameter | Units | Description | BODC Parameter Code | Units | Comments |
|---|---|---|---|---|---|
| Chl_a | mg m -3 | Concentration of chlorophyll-a {chl-a} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | CPHLHPP1 | mg m -3 | n/a |
| Chl_a standard deviation | mg m -3 | Concentration standard deviation of chlorophyll-a {chl-a} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | SDCLHPP1 | mg m -3 | n/a |
| DV_Chl_a | mg m -3 | Concentration of divinyl chlorophyll-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DVCAHPP1 | ng l -1 | Unit conversion x1000 applied |
| DV_Chl_a standard deviation | mg m -3 | Concentration standard deviation of divinyl chlorophyll-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DVCASDP1 | ng l -1 | Unit conversion x1000 applied |
| Chlide_a | mg m -3 | Concentration of chlorophyllide-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | CIDAHPP1 | ng l -1 | Unit conversion x1000 applied |
| Chlide_a standard deviation | mg m -3 | Concentration standard deviation of chlorophyllide-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | CIDASDP1 | ng l -1 | Unit conversion x1000 applied |
| Chl_b | mg m -3 | Concentration of chlorophyll-b {chl-b} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | CHLBHPP1 | ng l -1 | Unit conversion x1000 applied |
| Chl_b standard deviation | mg m -3 | Concentration standard deviation of chlorophyll-b {chl-b} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | CHLBSDP1 | ng l -1 | Unit conversion x1000 applied |
| DV_Chl_b | mg m -3 | Concentration of divinyl chlorophyll-b per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DVCBHPP1 | ng l -1 | Unit conversion x1000 applied |
| DV_Chl_b standard deviation | mg m -3 | Concentration standard deviation of divinyl chlorophyll-b per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DVCBSDP1 | ng l -1 | Unit conversion x1000 applied |
| Chl_c1c2 | mg m -3 | Concentration of chlorophyll-c1c2 {chl-c1c2} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | C1C2HPP1 | ng l -1 | Unit conversion x1000 applied |
| Chl_c1c2 standard deviation | mg m -3 | Concentration standard deviation of chlorophyll-c1c2 {chl-c1c2} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | C1C2SDP1 | ng l -1 | Unit conversion x1000 applied |
| Chl_c3 | mg m -3 | Concentration of chlorophyll-c3 {chl-c3} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | CLC3HPP1 | ng l -1 | Unit conversion x1000 applied |
| Chl_c3 standard deviation | mg m -3 | Concentration standard deviation of chlorophyll-c3 {chl-c3} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | CLC3SDP1 | ng l -1 | Unit conversion x1000 applied |
| alpha-beta-Car | mg m -3 | Concentration of alpha,beta-carotene {beta,epsilon-carotene} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | ABCRHPP1 | ng l -1 | Unit conversion x1000 applied |
| alpha-beta-Car standard deviation | mg m -3 | Concentration standard deviation of alpha,beta-carotene {beta,epsilon-carotene} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | ABCRSDP1 | ng l -1 | Unit conversion x1000 applied |
| But-fuco | mg m -3 | Concentration of 19'-butanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | BUTAHPP1 | ng l -1 | Unit conversion x1000 applied |
| But-fuco standard deviation | mg m -3 | Concentration standard deviation of 19'-butanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | BUTASDP1 | ng l -1 | Unit conversion x1000 applied |
| Hex-fuco | mg m -3 | Concentration of 19'-hexanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | HEXOHPP1 | ng l -1 | Unit conversion x1000 applied |
| Hex-fuco standard deviation | mg m -3 | Concentration standard deviation of 19'-hexanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | HEXOSDP1 | ng l -1 | Unit conversion x1000 applied |
| Allo | mg m -3 | Concentration of alloxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | ALLOHPP1 | ng l -1 | Unit conversion x1000 applied |
| Allo standard deviation | mg m -3 | Concentration standard deviation of alloxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | ALLOSDP1 | ng l -1 | Unit conversion x1000 applied |
| Diadino | mg m -3 | Concentration of diadinoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DIADHPP1 | ng l -1 | Unit conversion x1000 applied |
| Diadino standard deviation | mg m -3 | Concentration standard deviation of diadinoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DIADSDP1 | ng l -1 | Unit conversion x1000 applied |
| Diato | mg m -3 | Concentration of diatoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DIATHPP1 | ng l -1 | Unit conversion x1000 applied |
| Diato standard deviation | mg m -3 | Concentration standard deviation of diatoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | DIATSDP1 | ng l -1 | Unit conversion x1000 applied |
| Fuco | mg m -3 | Concentration of fucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | FUCXHPP1 | ng l -1 | Unit conversion x1000 applied |
| Fuco standard deviation | mg m -3 | Concentration standard deviation of fucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | FUCXSDP1 | ng l -1 | Unit conversion x1000 applied |
| Perid | mg m -3 | Concentration of peridinin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PERIHPP1 | ng l -1 | Unit conversion x1000 applied |
| Perid standard deviation | mg m -3 | Concentration standard deviation of peridinin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PERISDP1 | ng l -1 | Unit conversion x1000 applied |
| Zea | mg m -3 | Concentration of zeaxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | ZEOXHPP1 | ng l -1 | Unit conversion x1000 applied |
| Zea standard deviation | mg m -3 | Concentration standard deviation of zeaxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | ZEOXSDP1 | ng l -1 | Unit conversion x1000 applied |
| Lut | mg m -3 | Concentration of lutein per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | LUTNHPP1 | ng l -1 | Unit conversion x1000 applied |
| Lut standard deviation | mg m -3 | Concentration standard deviation of lutein per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | LUTNSDP1 | ng l -1 | Unit conversion x1000 applied |
| Neo | mg m -3 | Concentration of neoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | NEOXHPP1 | ng l -1 | Unit conversion x1000 applied |
| Neo standard deviation | mg m -3 | Concentration standard deviation of neoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | NEOXSDP1 | ng l -1 | Unit conversion x1000 applied |
| Viola | mg m -3 | Concentration of violaxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | VILXHPP1 | ng l -1 | Unit conversion x1000 applied |
| Viola standard deviation | mg m -3 | Concentration standard deviation of violaxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | VILXSDP1 | ng l -1 | Unit conversion x1000 applied |
| Phytin_a | mg m -3 | Concentration of phaeophytin-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PTAXHPP1 | ng l -1 | Unit conversion x1000 applied |
| Phytin_a standard deviation | mg m -3 | Concentration standard deviation of phaeophytin-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PTAXSDP1 | ng l -1 | Unit conversion x1000 applied |
| Phide_a | mg m -3 | Concentration of phaeophorbide-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PBAXHPP1 | ng l -1 | Unit conversion x1000 applied |
| Phide_a standard deviation | mg m -3 | Concentration standard deviation of phaeophorbide-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PBAXSDP1 | ng l -1 | Unit conversion x1000 applied |
| Pras | mg m -3 | Concentration of prasinoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PRSXHPP1 | ng l -1 | Unit conversion x1000 applied |
| Pras standard deviation | mg m -3 | Concentration standard deviation of prasinoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | PRSXSDP1 | ng l -1 | Unit conversion x1000 applied |
| Gyro | mg m -3 | Concentration of gyroxanthin diester per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | GDHPLC01 | ng l -1 | Unit conversion x1000 applied |
| Gyro standard deviation | mg m -3 | Concentration standard deviation of gyroxanthin diester per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) | GDIESDP1 | ng l -1 | Unit conversion x1000 applied |
Data Quality Report
The data originator also confirmed that values provided in the file of 0.001 mg m -3 were the detection limit. All these values have been flagged accordingly with the BODC flag for data below the detection limit.
BODC were not advised by the data originator of any further QC having taken place.
Problem Report
This section is not relevant to this data set.
