Metadata Report for BODC Series Reference Number 1637778
Metadata Summary
Problem Reports
Data Access Policy
Narrative Documents
Project Information
Data Activity or Cruise Information
Fixed Station Information
BODC Quality Flags
SeaDataNet Quality Flags
Metadata Summary
Data Description |
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Problem Reports
No Problem Report Found in the Database
Data Access Policy
Public domain data
These data have no specific confidentiality restrictions for users. However, users must acknowledge data sources as it is not ethical to publish data without proper attribution. Any publication or other output resulting from usage of the data should include an acknowledgment.
The recommended acknowledgment is
"This study uses data from the data source/organisation/programme, provided by the British Oceanographic Data Centre and funded by the funding body."
Narrative Documents
Non-toxic (underway) sea water supply
A source of uncontaminated near-surface (commonly 3 to 7 m) seawater pumped continuously to shipboard laboratories on research vessels. There is typically a temperature sensor near the intake (known as the hull temperature) to provide measurements that are as close as possible to the ambient water temperature. The flow from the supply is typically directed through continuously logged sensors such as a thermosalinograph and a fluorometer. Water samples are often collected from the non-toxic supply. The system is also referred to as the underway supply.
Pigments for cruise Poseidon PS211
Document History
Converted from CDROM documentation.
Content of data series
ACARHPP1 | Alpha-carotene |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
ALLOHPP1 | Alloxanthin |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
BCARHPP1 | Beta-carotene |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
BUTAHPP1 | Butanoyloxyfucoxanthin |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
C1C2HPP1 | Chlorophyll-c1c2 |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
CAROSSP1 | Spectrophotometric carotenoid pigments (SCOR) |
Spectrophotometric assay of acetone extraction (GF/F filtered) | |
milligrams/cubic metre | |
CHLBHPP1 | Chlorophyll-b |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
CHLBSSP1 | Spectrophotometric chlorophyll-b (SCOR) |
Spectrophotometric assay of acetone extraction (GF/F filtered) | |
Nanograms per litre | |
CHLCSSP1 | Spectrophotometric chlorophyll-c (SCOR) |
Spectrophotometric assay of acetone extraction (GF/F filtered) | |
Nanograms per litre | |
CLC3HPP1 | Chlorophyll-c3 |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
CLPHHPP1 | Chlorophyll-a plus phaeophorbides |
HPLC assay of acetone extract (GF/F filtered) | |
milligrams/cubic metre | |
CPHLFLP1 | Fluorometric chlorophyll-a |
Fluorometric assay of acetone extract (GF/F filtered) | |
Milligrams/cubic metre | |
CPHLFLP4 | Fluorometric chlorophyll-a |
Fluorometric assay of acetone extraction (sum of size fractions >0.2 microns) | |
Milligrams/cubic metre | |
CPHLFMP1 | Fluorometric chlorophyll-a |
Fluorometric assay of methanol extract (GF/F filtered) | |
Milligrams/cubic metre | |
CPHLHPP1 | HPLC chlorophyll-a |
HPLC assay of acetone extract (GF/F filtered) | |
Milligrams/cubic metre | |
CPHLPR01 | CTD chlorophyll |
Calibrated in-situ fluorometer | |
Milligrams/cubic metre | |
CPHLPRTX | Bench fluorometer chlorophyll |
Bench fluorometer measurement on unfiltered water sample | |
Milligrams/cubic metre | |
CPHLSPP1 | Spectrophotometric chlorophyll-a (Lorenzen) |
Spectrophotometric assay of acetone extraction (GF/F filtered) | |
Milligrams/cubic metre | |
CPHLSSP1 | Spectrophotometric chlorophyll-a (SCOR) |
Spectrophotometric assay of acetone extraction (GF/F filtered) | |
Milligrams/cubic metre | |
CPHLSSPC | Spectrophotometric chlorophyll-a (SCOR) |
Spectrophotometric assay of acetone extraction (centrifuged) | |
Milligrams/cubic metre | |
DIADHPP1 | Diadinoxanthin |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
DVCAHPP1 | Diavinyl chlorophyll-a |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
FUCXHPP1 | Fucoxanthin |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
HEXOHPP1 | Hexanoyloxyfucoxanthin |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
LUTNHPP1 | Lutein |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
PBA1HPP1 | Phaeophorbide-a1 |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
PBA2HPP1 | Phaeophorbide-a2 |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
PBA3HPP1 | Phaeophorbide-a3 |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
PERIHPP1 | Peridinin |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
PHAEFLP1 | Fluorometric phaeopigments |
Fluorometric assay of acetone extract (GF/F filtered) | |
Milligrams/cubic metre | |
PHAEFLP4 | Fluorometric phaeopigments |
Fluorometric assay of acetone extract (sum of size fractions >0.2 microns) | |
Milligrams/cubic metre | |
PHAEFMP1 | Fluorometric phaeopigments |
Fluorometric assay of methanol extract (GF/F filtered) | |
Milligrams/cubic metre | |
PHAESPP1 | Spectrophotometric phaeopigments (Lorenzen) |
Spectrophotometric assay of acetone extract (GF/F filtered) | |
Milligrams/cubic metre | |
PTA1HPP1 | Phaeophytin-a1 |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
PTA2HPP1 | Phaeophytin-a2 |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre | |
SCHLFLPA | Size-fractionated fluorometric chlorophyll-a |
Fluorometric assay of acetone extract (>5 micron size fraction) | |
Milligrams/cubic metre | |
SCHLFLPB | Size-fractionated fluorometric chlorophyll-a |
Fluorometric assay of acetone extract (>2 micron size fraction) | |
Milligrams/cubic metre | |
SCHLFLPC | Size-fractionated fluorometric chlorophyll-a |
Fluorometric assay of acetone extract (2-5 micron size fraction) | |
Milligrams/cubic metre | |
SCHLFLPF | Size-fractionated fluorometric chlorophyll-a |
Fluorometric assay of acetone extract (0.2-2 micron size fraction) | |
Milligrams/cubic metre | |
SPHAFLPA | Size-fractionated phaeopigments |
Fluorometric assay of acetone extract (>5 micron size fraction) | |
Milligrams/cubic metre | |
SPHAFLPB | Size-fractionated phaeopigments |
Fluorometric assay of acetone extract (>2 micron size fraction) | |
Milligrams/cubic metre | |
SPHAFLPC | Size-fractionated phaeopigments |
Fluorometric assay of acetone extract (0.2-2 micron size fraction) | |
Milligrams/cubic metre | |
SPHAFLPF | Size-fractionated phaeopigments |
Fluorometric assay of acetone extract (>2 micron size fraction) | |
Milligrams/cubic metre | |
TCPEFLP1 | Total chloroplastic pigment |
Fluorometric assay of acetone extract (GF/F filtered) | |
Milligrams/cubic metre | |
ZEOXHPP1 | Zeoxantin |
HPLC assay of acetone extract (GF/F filtered) | |
Nanograms per litre |
Data Originator
Dr Stuart Gibb, Plymouth Marine Laboratory, UK.
Sampling strategy and methodology
Water samples were either collected from water bottles deployed on a CTD rosette, bottles deployed on a hydrographic wire or taken from a continuous surface seawater supply.
1-2 litres of water were filtered through a 25mm GF/F filter, flash frozen and stored in liquid nitrogen until analysed either on board or back in the laboratory.
Pigment concentrations were determined by reverse phase HPLC following the protocols described in Barlow et al. (1993a). Frozen filters were extracted in 90% acetone, sonicated and centrifuged to remove debris. An aliquot (300 µl) of clarified extract was mixed with an equal volume of 1M ammonium acetate and 100 µl of this mixture was injected into a Shimazdu HPLC system incorporating a 3 micron C18 Pecosphere column (3.3 x 0.45 cm, Perkin Elmer) heated to 30 °C.
Pigments were separated by a linear binary gradient changing from 0% B to 100% B over 10 minutes, followed by an isocratic hold at 100% B for 7.5 minutes, at a flow rate of 1 ml per minute. Solvent A consisted of 80:20 (v/v) MeOH : ammonium acetate. Solvent B contained 60:40 (v/v) MeOH : acetone.
Chlorophylls and carotenoids were detected by absorbance at 440nm and phaeopigments by fluorescence detection at 405nm excitation, 670nm emission. Data collection and integration was performed with the Philips PU6000 chromatography software. Diavynyl chlorophyll a was determined on some samples using a C8 column as described by Barlow et al. (1996).
Pigments were identified and calibrated by comparison with retention times of pigments isolated from well-documented microalgal species in the Plymouth Culture Collection and with standards obtained from the Water Quality Institute, Denmark. Peak identity was further confirmed on selected samples by on-line diode array visible spectroscopy. Chlorophyll a and b were calibrated using authentic standards (Sigma Chemical Co.) in acetone and quantified spectrophotometrically using the extinction coefficients of Jeffrey and Humphrey (1975). Diavynyl chlorophyll a standard was obtained from R. Bidigare, University of Hawaii. Phaeopigment concentrations were estimated from peak areas and calibrations performed by simultaneous absorbance (667nm) and fluorescence detection of phaeopigments extracted from copepod and mussel faeces as detailed by Barlow et al. (1993b).
All pigments were supplied in units of ng/l. Chlorophyll a values were converted to mg/m3 by dividing by 1000 to unify units for this parameter in the database.
Comments on data quality
RRS Discovery DI217
The CTD pressure sensor gave rise to problems during this cruise and for the first three stations only wire out data were available. Whilst every effort has been made to correct the pressure channel and accurately match bottle firing pressures, the possibility for error should be borne in mind by users of the data.
References
Barlow, R.G., Mantoura, R.F.C., Gough, M.A. and Fileman, T.W., 1993a. Pigment signatures of the phytoplankton composition in the north-east Atlantic during the 1990 spring bloom. Deep Sea Res. II, 40, 459-477.
Barlow, R.G., Mantoura, R.F.C., Gough, M.A. and Fileman, T.W., 1993b. Phaeopigment distribution during the 1990 spring bloom in the north-east Atlantic. Deep Sea Res. I, 40, 2229-2242.
Barlow, R.G., Cummings, D.G., Mantoura, R.F.C. and Fileman, T.W., 1996. Pigment chemotaxonomic distributions of phytoplankton during summer in the western Mediterranean. Deep Sea Res. II, in press.
Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W. and Strictland, J.D.H., 1965. Fluorometric determination of chlorophyll. J. Con. perm. int. Explor. 30, 3-15.
Jeffrey, S.W. and Humphrey, G.F., 1975. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflan., 167, 191-194.
Lorenzen, C.J., 1967. Determination of chlorophyll and phaeopigments: spectrophotometric equations. Limnology and Oceanography, 12.
Lorenzen, C.J. and Jeffrey, S.W., 1978. Determination of chlorophyll in seawater. UNESCO Techn. Paper Mar Sci, 35.
Strickland, J.D.H., Parsons, T.R. (1972). A practical handbook of seawater analysis. Fish. Res. Bd. Can.,.167-311.
Tahey, T.M., Duineveld, G.C.A., Berghuis, E.M. and Helder, W., 1994. Relation between sediment-water fluxes of oxygen and silicate and faunal abundance at continental shelf, slope and deep-water stations in the North West Mediterranean. Marine Ecology Progress Series, 104, 119-130.
Thomsen. L., Graf, G., Martens, V. and Steen, E., 1994. An instrument for sampling water from the bottom nepheloid layer. Contin. Shelf Res., 14, 871-882.
Thomsen, L. and Graf, G., 1995. Benthic boundary layer characteristics of the continental margin of the western Barents Sea. Oceanologica Acta, 17/6, 597-607.
Wright, S.W., Jeffrey, S.W., Mantoura, R.F.C., Llewellyn, C.A., Bjornland, T., Repeta, D. and Welschmeyer, N., 1991. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series, 77, 183-196.
Project Information
Ocean Margin EXchange (OMEX) I
Introduction
OMEX was a European multidisciplinary oceanographic research project that studied and quantified the exchange processes of carbon and associated elements between the continental shelf of western Europe and the open Atlantic Ocean. The project ran in two phases known as OMEX I (1993-1996) and OMEX II - II (1997-2000), with a bridging phase OMEX II - I (1996-1997). The project was supported by the European Union under the second and third phases of its MArine Science and Technology Programme (MAST) through contracts MAS2-CT93-0069 and MAS3-CT97-0076. It was led by Professor Roland Wollast from Université Libre de Bruxelles, Belgium and involved more than 100 scientists from 10 European countries.
Scientific Objectives
The aim of the Ocean Margin EXchange (OMEX) project was to gain a better understanding of the physical, chemical and biological processes occurring at the ocean margins in order to quantify fluxes of energy and matter (carbon, nutrients and other trace elements) across this boundary. The research culminated in the development of quantitative budgets for the areas studied using an approach based on both field measurements and modeling.
OMEX I (1993-1996)
The first phase of OMEX was divided into sub-projects by discipline:
- Physics
- Biogeochemical Cycles
- Biological Processes
- Benthic Processes
- Carbon Cycling and Biogases
This emphasises the multidisciplinary nature of the research.
The project fieldwork focussed on the region of the European Margin adjacent to the Goban Spur (off the coast of Brittany) and the shelf break off Tromsø, Norway. However, there was also data collected off the Iberian Margin and to the west of Ireland. In all a total of 57 research cruises (excluding 295 Continuous Plankton Recorder tows) were involved in the collection of OMEX I data.
Data Availability
Field data collected during OMEX I have been published by BODC as a CD-ROM product, entitled:
- OMEX I Project Data Set (two discs)
Further descriptions of this product and order forms may be found on the BODC web site.
The data are also held in BODC's databases and subsets may be obtained by request from BODC.
Data Activity or Cruise Information
Cruise
Cruise Name | PO211 |
Departure Date | 1995-08-31 |
Arrival Date | 1995-09-11 |
Principal Scientist(s) | Ludger Mintrop (Institute of Marine Sciences, Kiel) |
Ship | FS Poseidon |
Complete Cruise Metadata Report is available here
Fixed Station Information
No Fixed Station Information held for the Series
BODC Quality Control Flags
The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:
Flag | Description |
---|---|
Blank | Unqualified |
< | Below detection limit |
> | In excess of quoted value |
A | Taxonomic flag for affinis (aff.) |
B | Beginning of CTD Down/Up Cast |
C | Taxonomic flag for confer (cf.) |
D | Thermometric depth |
E | End of CTD Down/Up Cast |
G | Non-taxonomic biological characteristic uncertainty |
H | Extrapolated value |
I | Taxonomic flag for single species (sp.) |
K | Improbable value - unknown quality control source |
L | Improbable value - originator's quality control |
M | Improbable value - BODC quality control |
N | Null value |
O | Improbable value - user quality control |
P | Trace/calm |
Q | Indeterminate |
R | Replacement value |
S | Estimated value |
T | Interpolated value |
U | Uncalibrated |
W | Control value |
X | Excessive difference |
SeaDataNet Quality Control Flags
The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:
Flag | Description |
---|---|
0 | no quality control |
1 | good value |
2 | probably good value |
3 | probably bad value |
4 | bad value |
5 | changed value |
6 | value below detection |
7 | value in excess |
8 | interpolated value |
9 | missing value |
A | value phenomenon uncertain |
B | nominal value |
Q | value below limit of quantification |