Metadata Report for BODC Series Reference Number 1686723

Metadata Summary

Data Description

Data Category Water sample data
Instrument Type
Technicap NOEX bottle  discrete water samplers
Instrument Mounting lowered unmanned submersible
Originating Country United Kingdom
Originator Dr Karen Wild-Allen
Originating Organization Napier University School of Life Sciences (now Edinburgh Napier University School of Life, Sport & Social Sciences)
Processing Status banked
Online delivery of data Download available - Ocean Data View (ODV) format
Project(s) Provess

Data Identifiers

Originator's Identifier PE136_CTD_PIGX_133:CTD008
BODC Series Reference 1686723

Time Co-ordinates(UT)

Start Time (yyyy-mm-dd hh:mm) 1999-04-01 20:28
End Time (yyyy-mm-dd hh:mm) -
Nominal Cycle Interval -

Spatial Co-ordinates

Latitude 52.29821 N ( 52° 17.9' N )
Longitude 4.07539 E ( 4° 4.5' E )
Positional Uncertainty Unspecified
Minimum Sensor or Sampling Depth 1.6 m
Maximum Sensor or Sampling Depth 18.0 m
Minimum Sensor or Sampling Height 3.7 m
Maximum Sensor or Sampling Height 20.1 m
Sea Floor Depth 21.7 m
Sea Floor Depth Source -
Sensor or Sampling Distribution Unspecified -
Sensor or Sampling Depth Datum Unspecified -
Sea Floor Depth Datum Instantaneous - Depth measured below water line or instantaneous water body surface


BODC CODERankUnitsTitle
ADEPZZ011MetresDepth (spatial coordinate) relative to water surface in the water body
BOTTFLAG1Not applicableSampling process quality flag (BODC C22)
CAROSSP11Milligrams per cubic metreConcentration of carotenoid pigments per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and spectrophotometry and processed following SCOR protocol
CHLBSSP11Nanograms per litreConcentration of chlorophyll-b {chl-b CAS 519-62-0} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and trichromatic spectrophotometry following the Jeffrey and Humphrey protocol
CHLCSSP11Nanograms per litreConcentration of chlorophyll-c {chl-c} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and trichromatic spectrophotometry following the Jeffrey and Humphrey protocol
CPHLSPP11Milligrams per cubic metreConcentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and spectrophotometry and processing following the Lorenzen protocol
CPHLSSP11Milligrams per cubic metreConcentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and trichromatic spectrophotometry following the Jeffrey and Humphrey protocol
PHAESPP11Milligrams per cubic metreConcentration of phaeopigments {pheopigments} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and spectrophotometry and processing following the Lorenzen protocol
SAMPRFNM1DimensionlessSample reference number

Definition of BOTTFLAG

0The sampling event occurred without any incident being reported to BODC.
1The filter in an in-situ sampling pump physically ruptured during sample resulting in an unquantifiable loss of sampled material.
2Analytical evidence (e.g. surface water salinity measured on a sample collected at depth) indicates that the water sample has been contaminated by water from depths other than the depths of sampling.
3The feedback indicator on the deck unit reported that the bottle closure command had failed. General Oceanics deck units used on NERC vessels in the 80s and 90s were renowned for reporting misfires when the bottle had been closed. This flag is also suitable for when a trigger command is mistakenly sent to a bottle that has previously been fired.
4During the sampling deployment the bottle was fired in an order other than incrementing rosette position. Indicative of the potential for errors in the assignment of bottle firing depth, especially with General Oceanics rosettes.
5Water was reported to be escaping from the bottle as the rosette was being recovered.
6The bottle seals were observed to be incorrectly seated and the bottle was only part full of water on recovery.
7Either the bottle was found to contain no sample on recovery or there was no bottle fitted to the rosette position fired (but SBE35 record may exist).
8There is reason to doubt the accuracy of the sampling depth associated with the sample.
9The bottle air vent had not been closed prior to deployment giving rise to a risk of sample contamination through leakage.

Definition of Rank

  • Rank 1 is a one-dimensional parameter
  • Rank 2 is a two-dimensional parameter
  • Rank 0 is a one-dimensional parameter describing the second dimension of a two-dimensional parameter (e.g. bin depths for moored ADCP data)

Problem Reports

No Problem Report Found in the Database

Data Access Policy

Public domain data

These data have no specific confidentiality restrictions for users. However, users must acknowledge data sources as it is not ethical to publish data without proper attribution. Any publication or other output resulting from usage of the data should include an acknowledgment.

The recommended acknowledgment is

"This study uses data from the data source/organisation/programme, provided by the British Oceanographic Data Centre and funded by the funding body."

Narrative Documents

Technicap NOEX bottle

The Technicap NOEX water bottle was designed for oceanographic sampling. The bottle comprises a plastic tube with caps at each end and is deployed with the caps held open, then closed at the target depth. Multiple bottles can be deployed on a CTD frame rosette. Bottle capacity is variable, but normally several litres. The bottle was developed by Technicap in the 1990s but has been superseded by more recent models. There is no known source of support available for this sampler.

Phytoplankton pigment concentration data series for cruise Pelagia PE136

Document History

Converted from CDROM documentation

Data Originators

Paul Tett, Napier University, Edinburgh, UK.
Karen Wild-Allen, Napier University, Edinburgh, UK.

Content of data series

Parameter Unit Parameter code Number of samples Number of stations Comments
Chl.a (Lorenzen) µg l-1 CPHLSPP1 100 35 CTD + 14 NT + 1 BUCKET none
Phaeop.a (Lorenzen) µg l-1 PHAESPP1 100 as above none
Chl.a (SCOR) µg l-1 CPHLSSP1 100 as above none
Chl.b (SCOR) ng l-1 CHLBSSP1 100 as above none
Chl.c (SCOR) ng l-1 CHLCSSP1 100 as above none
Carotenoids (SCOR) µg l-1 CAROSSP1 100 as above none
Chl.a >5 µm (Lorenzen) µg l-1 SCHLSPPA 23 7 CTD + 1 BUCKET none
Phaeop.a >5 µm (Lorenzen) µg l-1 SPHASPPA 23 as above none
Chl.a >5 µm (SCOR) µg l-1 SCHLSSPA 23 as above none
Chl.b >5 µm (SCOR) ng l-1 SCHBSSPA 23 as above none
Chl.c >5 µm (SCOR) ng l-1 SCHCSSPA 23 as above none
Carotenoids >5 µm (SCOR) µg l-1 SCARSSPA 23 as above none
Chl.a <5 µm (Lorenzen) µg l-1 SCHLSPPM 23 7 CTD + 1 BUCKET none
Phaeop.a <5 µm (Lorenzen) µg l-1 SPHASPPM 23 as above none
Chl.a <5 µm (SCOR) µg l-1 SCHLSSPM 23 as above none
Chl.b <5 µm (SCOR) ng l-1 SCHBSSPM 23 as above none
Chl.c <5 µm (SCOR) ng l-1 SCHCSSPM 23 as above none
Carotenoids <5 µm (SCOR) µg l-1 SCARSSPM 23 as above none

CTD= CTD-Rosette water column sampling station.
NT= surface underway sampling from ship's non-toxic supply.

Sampling strategy and methodology

Water samples were mainly collected using the CTD-rosette water sampler. Samples were typically taken from the near-surface for comparison with optical measurements and from 1 to 2 additional depths in the water column. Additional samples were occasionally taken at the surface with a bucket and from the ship's non-toxic sea water supply.

Samples were filtered under vacuum through Micropore membrane (5 µm) and/or Whatman glass fibre filters (GF/F 0.7 µm). Filters were then placed in labelled test-tubes and stored in a dark container with dessicant in the freezer. Following the cruise the samples were transferred to the laboratory freezer to await extraction and analysis (at the University of Wales, Bangor).

Pigments on the filters were extracted in 10 ml 90% analar acetone (10% miliQ water, buffered with sodium bicarbonate), stored in a dark container and refrigerated for 24 hours. Extracted samples were centrifuged at 10,000 rpm for 10 minutes and decanted into 1 cm pathlength micro-cuvettes. The Shumidzu spectrophotometer holds 2 cuvettes and is initialised by measuring the optical density of 2 blanks (cuvettes filled with 90% acetone) in 0.5 nm bandwidths from 345 - 755 nm. This generates a 'baseline' against which samples are compared. Sample optical densities were measured (in 0.5 nm bandwidths from 345 - 755 nm) by replacing 1 blank with a sample-filled cuvette. To evaluate pheopigment concentration the samples were acidified with 3 drops of 2 molar hydrochloric acid and measured again.

Pigment concentrations can be calculated from spectra by comparing the ratios of absorption at various wavelengths. Equations for each pigment are given in µg l-1.

Lorenzen method:

BODC image

Trichromatic method with Jeffrey and Humphrey (1975) coefficients:

BODC image


BODC image
where: A is the pigment coefficient (11 µg cm ml-1)
R is the ratio of chlorophyll.a to pheopigment absorbance at 665 nm (1.7)
OD665 is the optical density at 665 nm relative to 750 nm
OD665A is the optical density of the acidified sample at 665 nm relative to 750 nm
E is the extract volume (ml)
V is the sample volume filtered (l)
p is the cuvette pathlength (cm).

BODC processing

In order to standardise parameter units with that held in BODC's parameter dictionary, chlorophyll b and c concentrations were converted from µg l-1 to ng l-1 by multiplying their original values by 1000. The rest of the data were loaded into a database under the ORACLE Relational Database Management System without modification.

Comments on data quality

None to report.


Jeffrey SW, Humphrey SF (1975) New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and phytoplankton. Biochem. Biophysiol. Planzen, 167, 191-194.

Project Information

PROcesses of Vertical Exchange in Shelf Seas (PROVESS)


PROVESS was an interdisciplinary study of the vertical fluxes of properties through the water column and the surface and bottom boundary layers. The project was funded by the European Community MAST-III programme (MAS3-CT97- 0159) and ran from March 1998 to May 2001.

Scientific Rationale

PROVESS was based on the integration of experimental, theoretical and modelling studies with the aim of improving understanding and quantification of vertical exchange processes in the water column, in particular in the surface and benthic boundary layers and across the> pycnocline. PROVESS also explored mechanisms of physical-biological coupling in which vertical exchanges and turbulence significantly affect the environmental conditions experienced by the biota with particular reference to aggregation, flocculation, sedimentation and trophic interactions.


The experimental phase of the project was carried out at two contrasting sites in the North Sea: the northern North Sea site (NNS) and the southern North Sea site (SNS).

The two sites had the following characteristics:

Position 52° 15.0' N, 4° 17.0' E 59° 20.0' E, 1° 00.0' E
Time of year April-May September-November
Water depth (m) 16 100
M2 max amplitude (m s-1) 0.75 0.15
Max current (m s-1) 1.0 0.6
Delta T (deg C) mixed 7-1
Thermocline depth (m) mixed 35-100
Delta S 1 small
Halocline depth (m) 5-10 cf. thermocline depth
Max wind speed (m s-1) 20 25
Max wave height (m) 5 10
Max wave period (s) 8 10
Internal motion No Yes
Sediment muddy-sand muddy-sand
Biology eutrophic oligotrophic

At both locations measurements were concentrated at a central position with additional measurements being made to estimate horizontal gradients. Moored instruments (including current meters, temperature and pressure sensors, fluorometers, transmissometers, nutrient analysers and meteorological sensors) were deployed between 7 September and 5 November 1998 at the NNS and between 29 March and 25 May 1999 at the SNS. Each experiment was supported by intensive measurement series made from oceanographic ships and involving turbulence dissipation profiler CTD, particle size profilers, optical profilers, benthic sampling and water bottle sampling.

Details of the cruises were as follows:

Site Ship
NNS Valdivia (GER) VA174 5 - 17 Sep 1998
  Dana (DK) D1198 14 - 26 Oct 1998
  Pelagia (NL) PE125 19 - 30 Oct 1998
  Challenger (UK) CH140 22 Oct - 9 Nov 1998
SNS Pelagia (NL) PE135 29 Mar - 9 Apr 1999
  Mitra (NL) MT0499 19 - 30 Apr 1999
  Belgica (BE) BG9912 17 - 21 May 1999

Data Activity or Cruise Information

Data Activity

Start Date (yyyy-mm-dd) 1999-04-01
End Date (yyyy-mm-dd) 1999-04-01
Organization Undertaking ActivityRoyal Netherlands Institute for Sea Research
Country of OrganizationNetherlands
Originator's Data Activity IdentifierPE136_CTD_CTD008
Platform Categorylowered unmanned submersible

BODC Sample Metadata Report for PE136_CTD_CTD008

Sample reference number Nominal collection volume(l) Bottle rosette position Bottle firing sequence number Minimum pressure sampled (dbar) Maximum pressure sampled (dbar) Depth of sampling point (m) Bottle type Sample quality flag Bottle reference Comments
382686   12.00       17.90   19.50   18.00 Technicap NOEX bottle No problem reported    
382687   12.00        1.30    2.90    1.60 Technicap NOEX bottle No problem reported    

Please note:the supplied parameters may not have been sampled from all the bottle firings described in the table above. Cross-match the Sample Reference Number above against the SAMPRFNM value in the data file to identify the relevant metadata.


Cruise Name PE136
Departure Date 1999-03-29
Arrival Date 1999-04-09
Principal Scientist(s)Hans van Haren (Royal Netherlands Institute for Sea Research)
Ship RV Pelagia

Complete Cruise Metadata Report is available here

Fixed Station Information

No Fixed Station Information held for the Series

BODC Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

Flag Description
Blank Unqualified
< Below detection limit
> In excess of quoted value
A Taxonomic flag for affinis (aff.)
B Beginning of CTD Down/Up Cast
C Taxonomic flag for confer (cf.)
D Thermometric depth
E End of CTD Down/Up Cast
G Non-taxonomic biological characteristic uncertainty
H Extrapolated value
I Taxonomic flag for single species (sp.)
K Improbable value - unknown quality control source
L Improbable value - originator's quality control
M Improbable value - BODC quality control
N Null value
O Improbable value - user quality control
P Trace/calm
Q Indeterminate
R Replacement value
S Estimated value
T Interpolated value
U Uncalibrated
W Control value
X Excessive difference

SeaDataNet Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

Flag Description
0 no quality control
1 good value
2 probably good value
3 probably bad value
4 bad value
5 changed value
6 value below detection
7 value in excess
8 interpolated value
9 missing value
A value phenomenon uncertain
Q value below limit of quantification