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Metadata Report for BODC Series Reference Number 1880594


Metadata Summary

Data Description

Data Category Water sample data
Instrument Type
NameCategories
Non-toxic sea water supply  continuous water samplers
Instrument Mounting research vessel
Originating Country United Kingdom
Originator Dr Alex Poulton
Originating Organization Southampton Oceanography Centre (now National Oceanography Centre, Southampton)
Processing Status banked
Online delivery of data Download available - Ocean Data View (ODV) format
Project(s) Atlantic Meridional Transect Phase2(AMT)
 

Data Identifiers

Originator's Identifier AMT14_GPUMP_PIGX_215:
BODC Series Reference 1880594
 

Time Co-ordinates(UT)

Start Time (yyyy-mm-dd hh:mm) 2004-04-29 22:09
End Time (yyyy-mm-dd hh:mm) 2004-05-30 12:15
Nominal Cycle Interval -
 

Spatial Co-ordinates

Southernmost Latitude 48.01987 S ( 48° 1.2' S )
Northernmost Latitude 49.25715 N ( 49° 15.4' N )
Westernmost Longitude 51.75972 W ( 51° 45.6' W )
Easternmost Longitude 6.62063 W ( 6° 37.2' W )
Positional Uncertainty 0.05 to 0.1 n.miles
Minimum Sensor or Sampling Depth 6.0 m
Maximum Sensor or Sampling Depth 6.0 m
Minimum Sensor or Sampling Height -
Maximum Sensor or Sampling Height -
Sea Floor Depth -
Sea Floor Depth Source -
Sensor or Sampling Distribution Unspecified -
Sensor or Sampling Depth Datum Unspecified -
Sea Floor Depth Datum Unspecified -
 

Parameters

BODC CODERankUnitsTitle
AADYAA011DaysDate (time from 00:00 01/01/1760 to 00:00 UT on day)
AAFDZZ011DaysTime (time between 00:00 UT and timestamp)
ALATGP011DegreesLatitude north relative to WGS84 by unspecified GPS system
ALLOHPP11Nanograms per litreConcentration of alloxanthin {CAS 28380-31-6} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
ALONGP011DegreesLongitude east relative to WGS84 by unspecified GPS system
BUTAHPP11Nanograms per litreConcentration of 19'-butanoyloxyfucoxanthin per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
CHLBHPP11Nanograms per litreConcentration of chlorophyll-b {chl-b CAS 519-62-0} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
CLC2HPP11Nanograms per litreConcentration of chlorophyll-c2 {chl-c2} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
CLC3HPP11Nanograms per litreConcentration of chlorophyll-c3 {chl-c3} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
CPHLFLP11Milligrams per cubic metreConcentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and fluorometry
CPHLHPP11Milligrams per cubic metreConcentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
DIADHPP11Nanograms per litreConcentration of diadinoxanthin {CAS 18457-54-0} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
DVCAHPP11Nanograms per litreConcentration of divinyl chlorophyll-a {DVchl-a} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
FUCXHPP11Nanograms per litreConcentration of fucoxanthin {CAS 3351-86-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
HEXOHPP11Nanograms per litreConcentration of 19'-hexanoyloxyfucoxanthin {CAS 60147-85-5} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
LUTNHPP11Nanograms per litreConcentration of lutein {CAS 127-40-2} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
PERIHPP11Nanograms per litreConcentration of peridinin {CAS 33281-81-1} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
PRSXHPP11Nanograms per litreConcentration of prasinoxanthin {CAS 94705-97-2} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
VILXHPP11Nanograms per litreConcentration of violaxanthin {CAS 126-29-4} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)
ZEOXHPP11Nanograms per litreConcentration of zeaxanthin {CAS 144-68-3} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

Definition of Rank

  • Rank 1 is a one-dimensional parameter
  • Rank 2 is a two-dimensional parameter
  • Rank 0 is a one-dimensional parameter describing the second dimension of a two-dimensional parameter (e.g. bin depths for moored ADCP data)

Problem Reports

No Problem Report Found in the Database


Data Access Policy

Open Data supplied by Natural Environment Research Council (NERC)

You must always use the following attribution statement to acknowledge the source of the information: "Contains data supplied by Natural Environment Research Council."


Narrative Documents

Non-toxic (underway) sea water supply

A source of uncontaminated near-surface (commonly 3 to 7 m) seawater pumped continuously to shipboard laboratories on research vessels. There is typically a temperature sensor near the intake (known as the hull temperature) to provide measurements that are as close as possible to the ambient water temperature. The flow from the supply is typically directed through continuously logged sensors such as a thermosalinograph and a fluorometer. Water samples are often collected from the non-toxic supply. The system is also referred to as the underway supply.

AMT14 extracted chlorophyll-a measured fluormetrically from CTD bottle and underway samples

Data Acquisition and Analysis

Size-fractionated measurements were only performed on samples collected from the CTD rosette sampler. Total chlorophyll was analysed on samples collected from both the CTD rosette sampler and the ship's non-toxic water supply. During AMT14 underway samples were collected every 2 hrs. Water-column sampling during AMT14 concentrated around 5 or 6 light depths from the predawn CTD cast (~0200 - 0400h local time), with a reduced set of depths from the late morning 'optics' cast (1100h local time).

Water samples from the 5-6 main light depths were collected from CTD bottles and underway samples from the ship's non-toxic supply and these samples were filtered on 25 mm GFF filters. Filters were extracted in 90% acetone for 10-24 hrs and total chlorophyll-a measured with a TD-700 Turner Designs fluorometer following the procedure of Welschmeyer (1994) which minimises interference by chlorophyll-b. The fluorometer was calibrated with dilutions of a solution of pure chlorophyll-a (Sigma, UK) in 90% acetone, the concentration of which was determined spectrophotometrically after the cruise.

Size-fractionation of chlorophyll was carried out at 29 stations on a total of 140 samples. Methodology for size-fractionation was not detailed in the AMT14 cruise report but was confirmed to be the same as that used for AMT13 by originator. Size-fractionated chlorophyll distribution was determined by serial filtration onto 10, 5, 2 and 0.2 µm polycarbonate membrane filters (Poretics, UK). The filters were treated identically to the GF/F used for total chlorophyll and were soaked in 10-ml 90% acetone (HPLC grade) for 10-24 hours prior to reading on the bench fluorometer.

References Cited

Welschmeyer N.A., 1994. Fluorometric analysis of chlorophyll-a in the presence of chlorophyll-b and phaeopigments. Limnology and Oceanography, 39, 1985-1992.

Instrumentation Description

Not relevant to this dataset.

BODC Data Processing Procedures

Data were submitted to BODC in 2 Excel spreadsheets. The CTD (including size-fractionated data) were accessioned under BODC archive SOC040122 and the underway samples under BODC archive SOC040147. Sample metadata provided (CTD cast, date, time, lat/lon and depth) were checked against information held in the database. There were 3 discrepancies noted and these have now been checked with the originator:

    - excessive difference in depth for station AMT14_06 between originator's depths and BODC's CTD depths: closest match to samples attributed to 100 and 150 m were 81.1 and 176.3 m respectively. Originator agreed with correction.
    - the surface sample from station AMT14_10 corresponds to a Niskin bottle reported as having misfired in UKORS' CTD log sheets. This was also observed with Mike Zubkov's AFC data. Following enquiry, originator confirmed that the sample did indeed originate from that particular bottle and that some water had been collected despite the misfiring problem.

Parameter codes defined in BODC parameter dictionary were assigned to the variable. The units supplied in the spreadsheet were mg m-3, which match the Parameter Dictionary units so no unit conversions were necessary.

Data loaded into BODC's database without any changes applied.

A parameter mapping table is provided below;

Originator's Parameter Units Description BODC Parameter Code Units Comments
Chlorophyll-a mg m-3 Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and fluorometry CPHLFLP1 mg m-3 n/a
0.2-2µm mg m-3 Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate 0.2-2µm phase] by filtration, acetone extraction and fluorometry SCHLFLPF mg m-3 n/a
2-5µm mg m-3 Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate 2-5µm phase] by filtration, acetone extraction and fluorometry SCHLFLPC mg m-3 n/a
5-10µm mg m-3 Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate 5-10µm phase] by filtration, acetone extraction and fluorometry SCHLFLPX mg m-3 n/a
>10µm mg m-3 Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate >10µm phase] by filtration, acetone extraction and fluorometry SCHLFLPO mg m-3 n/a
Total mg m-3 Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate >0.2µm phase] by filtration, acetone extraction, fluorometry and summation of size-fractionated values CPHLFLP4 mg m-3 n/a

Data Quality Report

The following values were reported as suspect by the originator and were flagged 'L' in the database:

    AMT14_38, 46m : value for 0.2-2µm size fraction ("Incorrect measurement")
    AMT14_63, 50m: value for >10µm size-fraction ("too high")
    AMT14_67, 57m: value for 0.2-2µm ("too low, bad measurement")
    AMT14_85, 45m: value for 0.2-2µm ("too low, not extracted properly")

Two values (AMT14_06, 176 m and AMT14_09, 7.6 m) were found suspect during the calibration of the CTD fluorometer and were flagged 'M' in the database.

Problem Report

Not relevant to this dataset.

AMT14 pigment composition by HPLC analysis from surface underway samples

Data Acquisition and Analysis

Seawater samples were collected every 2 hours from the surface underway supply. All samples and replicates were stored at -80 °C and were returned to the National Oceanography Centre, Southampton in liquid-N for analysis.

HPLC analysis was carried out according to Barlow et al. (1997).

References Cited

Barlow R.G., Cummings D.G. and Gibb S.W., 1997. Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C8 HPLC. Marine Ecology Progress Series, 161 , 303-307.

Instrumentation Description

Section not relevant.

BODC Data Processing Procedures

Data were submitted to BODC in Microsoft Excel spreadsheet format and saved to the archive with accession SOC050049. The file was provided to BODC with date, time, lat and lon as metadata. Sample metadata were checked against information held in the database - there were no discrepancies.

Parameter codes defined in BODC parameter dictionary were assigned to the variables as shown in the table below. Values were multiplied by one thousand where appropriate for conversion into nanograms per litre. Tchl data were provided by the originator, but were not loaded to the database as the data were derived values calculated as the sum of Chla and Dvchla values.

The parameter code mapping is shown in the table below:

Originator's Parameter Units Description BODC Parameter Code Units Comments
Chl c3 mg m-3 Concentration of chlorophyll-c3 {chl-c3} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) CLC3HPP1 ng l-1 Unit conversion x1000 applied
Chl c2 mg m-3 Concentration of chlorophyll-c2 {chl-c2} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) CLC2HPP1 ng l-1 Unit conversion x1000 applied
Peridinin mg m-3 Concentration of peridinin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) PERIHPP1 ng l-1 Unit conversion x1000 applied
19 But mg m-3 Concentration of 19'-butanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) BUTAHPP1 ng l-1 Unit conversion x1000 applied
Fuco mg m-3 Concentration of fucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) FUCXHPP1 ng l-1 Unit conversion x1000 applied
19Hex mg m-3 Concentration of 19'-hexanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) HEXOHPP1 ng l-1 Unit conversion x1000 applied
Prasino mg m-3 Concentration of prasinoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) PRSXHPP1 ng l-1 Unit conversion x1000 applied
Violoxan mg m-3 Concentration of violoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) VILXHPP1 ng l-1 Unit conversion x1000 applied
Diadino mg m-3 Concentration of diadinoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) DIADHPP1 ng l-1 Unit conversion x1000 applied
Alloxan mg m-3 Concentration of alloxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) ALLOHPP1 ng l-1 Unit conversion x1000 applied
Zeaxan mg m-3 Concentration of zeaxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) ZEOXHPP1 ng l-1 Unit conversion x1000 applied
Lutein mg m-3 Concentration of lutein per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) LUTNHPP1 ng l-1 Unit conversion x1000 applied
Chlb mg m-3 Concentration of chlorophyll-b per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) CHLBHPP1 ng l-1 Unit conversion x1000 applied
Dvchla mg m-3 Concentration of divinyl chlorophyll-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) DVCAHPP1 ng l-1 Unit conversion x1000 applied
Chl a mg m-3 Concentration of chlorophyll-a {chl-a} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC) CPHLHPP1 mg m-3 n/a

Data Quality Report

The originator quality checked the dataset and indicated data that were considered suspect. These data have been flagged accordingly in the database.

Problem Report

This section is not relevant to this data set.


Project Information

The Atlantic Meridional Transect - Phase 2 (2002-2006)

Who was involved in the project?

The Atlantic Meridional Transect Phase 2 was designed by and implemented by a number of UK research centres and universities. The programme was hosted by Plymouth Marine Laboratory in collaboration with the National Oceanography Centre, Southampton. The universities involved were:

  • University of Liverpool
  • University of Newcastle
  • University of Plymouth
  • University of Southampton
  • University of East Anglia

What was the project about?

AMT began in 1995, with scientific aims to assess mesoscale to basin scale phytoplankton processes, the functional interpretation of bio-optical signatures and the seasonal, regional and latitudinal variations in mesozooplankton dynamics. In 2002, when the programme restarted, the scientific aims were broadened to address a suite of cross-disciplinary questions concerning ocean plankton ecology and biogeochemistry and the links to atmospheric processes.

The objectives included the determination of:

  • how the structure, functional properties and trophic status of the major planktonic ecosystems vary in space and time
  • how physical processes control the rates of nutrient supply to the planktonic ecosystem
  • how atmosphere-ocean exchange and photo-degradation influence the formation and fate of organic matter

The data were collected with the aim of being distributed for use in the development of models to describe the interactions between the global climate system and ocean biogeochemistry.

When was the project active?

The second phase of funding allowed the project to continue for the period 2002 to 2006 and consisted of six research cruises. The first phase of the AMT programme ran from 1995 to 2000.

Brief summary of the project fieldwork/data

The fieldwork on the first three cruises was carried out along transects from the UK to the Falkland Islands in September and from the Falkland Islands to the UK in April. The last three cruises followed a cruise track between the UK and South Africa, only deviating from the traditional transect in the southern hemisphere. During this phase the research cruises sampled further into the centre of the North and South Atlantic Ocean and also along the north-west coast of Africa where upwelled nutrient rich water is known to provide a significant source of climatically important gases.

Who funded the project?

Natural Environment Research Council (NERC)


Data Activity or Cruise Information

Cruise

Cruise Name JR20040428 (AMT14, JR101)
Departure Date 2004-04-28
Arrival Date 2004-06-01
Principal Scientist(s)Patrick M Holligan (University of Southampton School of Ocean and Earth Science)
Ship RRS James Clark Ross

Complete Cruise Metadata Report is available here


Fixed Station Information


No Fixed Station Information held for the Series


BODC Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

Flag Description
Blank Unqualified
< Below detection limit
> In excess of quoted value
A Taxonomic flag for affinis (aff.)
B Beginning of CTD Down/Up Cast
C Taxonomic flag for confer (cf.)
D Thermometric depth
E End of CTD Down/Up Cast
G Non-taxonomic biological characteristic uncertainty
H Extrapolated value
I Taxonomic flag for single species (sp.)
K Improbable value - unknown quality control source
L Improbable value - originator's quality control
M Improbable value - BODC quality control
N Null value
O Improbable value - user quality control
P Trace/calm
Q Indeterminate
R Replacement value
S Estimated value
T Interpolated value
U Uncalibrated
W Control value
X Excessive difference

SeaDataNet Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

Flag Description
0 no quality control
1 good value
2 probably good value
3 probably bad value
4 bad value
5 changed value
6 value below detection
7 value in excess
8 interpolated value
9 missing value
A value phenomenon uncertain
Q value below limit of quantification