Metadata Report for BODC Series Reference Number 2052072

• Metadata Summary

• Problem Reports

• Data Access Policy

• Narrative Documents

• Project Information

• Data Activity or Cruise Information

• Fixed Station Information

• BODC Quality Flags

• SeaDataNet Quality Flags

Metadata Summary

Problem Reports

No Problem Report Found in the Database

Data Access Policy

Open Data supplied by Natural Environment Research Council (NERC)

You must always use the following attribution statement to acknowledge the source of the information: "Contains data supplied by Natural Environment Research Council."

Narrative Documents

Becton Dickinson FACScan flow cytometer

A flow cell-based benchtop cytometer. It uses hydrodynamic focusing to pass particles through a laser light. The scattered and fluorescent light is collected by appropriately positioned lenses. A combination of beam splitters and filters steers the light to the appropriate detectors. The detectors produce electronic signals proportional to the optical signals striking them. The analyser uses a 20mW Coherent 488nm, air cooled, argon ion laser. Detectors are three photomultiplier tubes with bandpass filters for FITC (530/30nm), PE (575/26nm), PerCP, or PE-Cy5,or PE-Cy5-5 or PE-Cy7 (650nmLP). Fluorescence sensitivity: <2,000 molecules of equivalent soluble fluoroscein. Fluorescence resolution: < 3% for PI labelled chick erythrocyte nuclei.

For more information, please see this document: https://www.bodc.ac.uk/data/documents/nodb/pdf/BectonDickinsonFACScan_specifications.pdf

Clean pumped sea water supply

The system comprises a precision echosounder (PES) fish attached to a clean, reinforced tube (typically composed of braided polyvinyl chloride (PVC)). The fish is designed to be towed alongside a moving ship at a depth of one to three metres and water is drawn through the system by a clean pump. The tube usually leads to a clean laboratory on board the vessel, inside which samples are drawn for analysis. The system is typically used for continuous, underway, clean sampling (e.g., trace metal studies) of near surface waters.

Microbial Phytoplankton abundance from water samples collected from a towed FISH on Cruise JC150

Originator's Protocol for Data Acquisition and Analysis

Acquisition description:

Sampling methodology

Unfiltered seawater samples were collected for flow cytometry analysis from the underway fish sampling surface transect to gain insight into the microbial community composition. In brief, 1.8 mL of unfiltered seawater sample collected under trace metal clean conditions were transferred to a 1 mL cyovial containing 20 uL of 50 % glutaraldehyde solution. The vial was then inverted 10 times to allow the bubble to mix the solutions together prior to storage in the dark at 4 °C for up to 12 hours before storage at -80 °C until analysis in the laboratory.

Analytical methodology

All samples were analysed using a BD FACScan flow cytometer.

First, samples were thawed. Then, 500 uL from each sample was stained with Sybr Green 1 DNA dye for 1 hour in the dark at room temperature and then analysed for 1 minute at a flow rate of 62.75 uL per minute. The remaining thawed sample was analysed unstained for 6 minutes at a flow rate of 62.75 uL per minute. Each sample was analysed once, there were no replicates. Data were stored in listmode format and analysed using CellQuest software.

Further information can be found in the JC150 cruise report.

BODC Data Processing Procedures

Data received were loaded into the BODC database using established BODC data banking procedures. A parameter mapping table is provided below:

Project Information

Zinc, iron and phosphorous co-limitation in the Ocean: ZIPLOc

ZIPLOc is an 3 year project that aims to measure how zinc and phosphorous control biological activity in the North Atlantic subtropical gyre using novel measurement techniques. The observations made will be further explored using the latest modelling techniques over decadal timescales and in other basins.

The research aims to make an improvement in our overall understanding of how subtropical gyre ecosystems respond to ongoing climate change.

The project is led by the University of Liverpool, Earth, Ocean and Ecological Sciences and is a collaboration with the University of Southampton, School of Ocean and Earth Science. The project received funding from the Natural Environmental Research Council and runs between January 2017 and February 2020.

Data Activity or Cruise Information

Cruise

Complete Cruise Metadata Report is available here

Fixed Station Information

No Fixed Station Information held for the Series

BODC Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

SeaDataNet Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle: