Cultured eukaryotic plankton respiration measured by oxygen consumption and Iodo-Nitro-Tetrazolium (INT) reduction methods during the REMAIN project

(1) University of East Anglia School of Environmental Sciences

This dataset is comprised of culture experiments examining the respiration of 5 marine eukaryotic plankton organisms (Thalassiosira pseudonana CCMP1080/5, Emiliania huxleyi RCC1217, Pleurochrysis carterae PLY-406, Scrippsiella sp. RCC1720, and Oxyrrhis marina CCMP1133/5). The organisms were cultured in media made up in filtered seawater that was collected from the continuous seawater supply system available in the laboratories of the Centre for Environment Fisheries and Aquaculture Science in Lowestoft, UK pumped from the North Sea. Experiments were run between December 2017 and March 2019. The experiments examined the toxic effect of the INT and the relationship between the reduction of INT and oxygen consumption. Respiration is determined as the decrease in dissolved oxygen after a dark incubation (measured by Winkler titration 'CRO2' and optical sensors), and INT reduction 'INTR' is determined by the INT reduction method (Garcia-Martin et al. 2019). The experiments ran for two days; the first day comprised of a time series incubation to determine the time when the rate of formazan production began to decrease (maximum INT incubation) in the cultured organism, and the second day determined the relationship between oxygen consumption and INT reduction. The data also include the time the samples were incubated for each measurement, the cell abundance, and the temperature of the incubation. Data were generated by E. Elena Garcia-Martin, Isabel Seguro, and Carol Robinson of the University of East Anglia as part of the NERC Discovery Science project 'Remineralisation of organic carbon by marine bacterioplankton (REMAIN)' (grant reference NE/R000956/1 active from December 01, 2017 to November 30, 2020), and the 'Marine bacterioplankton respiration: a critical unknown in global carbon budgets' project by The Leverhulme Trust (RPG-2017-089).