Published Data Library (PDL)

AMT20 (JC053) microbial community by AFC

Title

Abundance and composition of microbial plankton communities using flow cytometry on cruise AMT20 (JC053).

Author(s) Holland R.J. (1); Zubkov M.V. (1)

(1) National Oceanography Centre, Southampton

Subject biota, oceans
Abstract

This dataset consists of 676 cellular abundance measurements of different microbial populations collected during the Atlantic Meridional Transect programme cruise 20 (AMT20, JC053) between 14th October and 21st November 2010. The cruise track between UK and Chile passed through both North and South Atlantic gyres. This dataset is a contribution to the AMT time-series of core oceanographic measurements. Daily samples were collected at 10 to 12 depth in the upper 300m of the water column using a standard CTD rosette equipped with 24 x 20L Niskin bottles. Samples were fixed with paraformaldehyde (1% w/v final concentration) and stained with the nucleic acid dye SybrGreen I (Sigma-Aldrich, UK). Abundance of different microbial populations was determined according to light-scattering properties (90° or side light scatter, SSC), relative concentration of SYBR Green I stain per particle (green fluorescence; FL1, 530 ± 30 nm) and chlorophyll content (red fluorescence; FL3, >650 nm) using a FACSort instrument (Becton Dickinson, UK).

Temporal coverage 14 October 2010 - 21 November 2010
Spatial coverage Atlantic Ocean
File format Delimited
Language English
Discovery metadata record Link to the related European Directory of Marine Environmental Data (EDMED) record
Publisher British Oceanographic Data Centre - Natural Environment Research Council, UK
Publication date 16 April 2014
Digital Object Identifier (DOI) doi:10.5285/f7021d34-028d-70fc-e044-000b5de50f38
Short DOI doi:10/sc3
Citation text Holland R.J.; Zubkov M.V. (2014). Abundance and composition of microbial plankton communities using flow cytometry on cruise AMT20 (JC053). British Oceanographic Data Centre - Natural Environment Research Council, UK. doi:10/sc3.

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