Published Data Library (PDL)
AMT20 (JC053) microbial community by AFC
Abundance and composition of microbial plankton communities using flow cytometry on cruise AMT20 (JC053).
Holland R.J. (1);
Zubkov M.V. (1)
(1) National Oceanography Centre, Southampton
This dataset consists of 676 cellular abundance measurements of different microbial populations collected during the Atlantic Meridional Transect programme cruise 20 (AMT20, JC053) between 14th October and 21st November 2010. The cruise track between UK and Chile passed through both North and South Atlantic gyres. This dataset is a contribution to the AMT time-series of core oceanographic measurements. Daily samples were collected at 10 to 12 depth in the upper 300m of the water column using a standard CTD rosette equipped with 24 x 20L Niskin bottles. Samples were fixed with paraformaldehyde (1% w/v final concentration) and stained with the nucleic acid dye SybrGreen I (Sigma-Aldrich, UK). Abundance of different microbial populations was determined according to light-scattering properties (90° or side light scatter, SSC), relative concentration of SYBR Green I stain per particle (green fluorescence; FL1, 530 ± 30 nm) and chlorophyll content (red fluorescence; FL3, >650 nm) using a FACSort instrument (Becton Dickinson, UK).
|Temporal coverage||14 October 2010 - 21 November 2010|
|Spatial coverage||Atlantic Ocean|
|Discovery metadata record||Link to the related European Directory of Marine Environmental Data (EDMED) record|
|Publisher||British Oceanographic Data Centre - Natural Environment Research Council, UK|
|Publication date||16 April 2014|
|Digital Object Identifier (DOI)||doi:10.5285/f7021d34-028d-70fc-e044-000b5de50f38|
|Citation text||Holland R.J.; Zubkov M.V.(2014). Abundance and composition of microbial plankton communities using flow cytometry on cruise AMT20 (JC053). British Oceanographic Data Centre - Natural Environment Research Council, UK. doi:10/sc3.|