Published Data Library (PDL)

AMT20 (JC053) microbial community carbon fixation


CO2 fixation of dominant bacterial and eukaryotic groups for cruise AMT20 (JC053).

Author(s) Hartmann M. (1); Gomez-Pereira P. (1); Grob C. (2); Zubkov M.V. (1)

(1) National Oceanography Centre, Southampton; (2) University of Warwick, School of Life Sciences

Subject biota, oceans

This dataset consists of 100 measurements of group-specific carbon fixation collected during the Atlantic Meridional Transect programme cruise 20 (AMT20, JC053) between 14th October and 21st November 2010. The cruise track between UK and Chile passed through both North and South Atlantic gyres. Pre-dawn seawater samples were collected from 20 m depth and occasionally a second depth at the bottom of the thermocline in 20 L Niskin bottles attached to a standard conductivity-temperature-depth profiler. Seawater samples were spiked with trace metal-clean 14C radiolabelled sodium bicarbonate (DHI, Denmark). Samples were then incubated at ambient temperatures in a 6 L water tank illuminated by a warm white light-emitting diode array adjusted to a constant output of 500 µmol photons m-2 s-1 for 10h. Subsequently, different phytoplankton populations (namely Prochlorococcus and Synechococcus cyanobacteria as well as small (approx. 2µn;m) and large (approx. 3.1µn;m) plastidic protists) were sorted according to light-scattering properties (90° or side light scatter, SSC), relative concentration of SYBR Green I stain per particle (green fluorescence; FL1, 530 ± 30 nm), phycoerythrin-content (orange fluorescence; FL2, 580 ± 30 nm) and chlorophyll content (red fluorescence; FL3, >650 nm) using a FACSort instrument (Becton Dickinson, UK). Radio-assaying of samples was carried out using an ultra-low-level liquid scintillation counter (1220 Quantulus, Wallac).

Temporal coverage 14 October 2010 - 21 November 2010
Spatial coverage Atlantic Ocean
File format Delimited
Language English
Discovery metadata record Link to the related European Directory of Marine Environmental Data (EDMED) record
Publisher British Oceanographic Data Centre - Natural Environment Research Council, UK
Publication date 16 April 2014
Digital Object Identifier (DOI) doi:10.5285/f7021d34-028f-70fc-e044-000b5de50f38
Short DOI doi:10/sc5
Citation text Hartmann M.; Gomez-Pereira P.; Grob C.; Zubkov M.V. (2014). CO2 fixation of dominant bacterial and eukaryotic groups for cruise AMT20 (JC053). British Oceanographic Data Centre - Natural Environment Research Council, UK. doi:10/sc5.

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