Published Data Library (PDL)

AMT20 (JC053) microbial community mixotrophy


Influence of light on bacterivory of the smallest eukaryotes on cruise AMT20 (JC053).

Author(s) Hartmann M. (1); Grob C. (2); Zubkov M.V. (1)

(1) National Oceanography Centre, Southampton; (2) University of Warwick, School of Life Sciences

Subject biota, oceans

This dataset consists of 672 individual measurements of 35S methionine uptake of bacterial and protist populations during a pulse-chase labelling experiment collected during the Atlantic Meridional Transect programme cruise 20 (AMT20, JC053) between 14th October and 21st November 2010. Sampling was restricted to the South Atlantic gyre. Pre-dawn seawater samples were collected from 20 m depth in 20 L Niskin bottles attached to a standard conductivity-temperature-depth profiler. Experiments were set up for dark and light measurements in a dark room. Light incubation experiments were placed in a 6-L water tank illuminated by a warm white light-emitting diode (LED) array. Parallel dark incubations were done in a similar water tank but were isolated from light. All experiments were run at ambient temperatures controlled by refrigerated water bath. To 250 mL seawater sample 0.4 nM L-[35S]methionine and 0.5 nM L-[4,5-3H]leucine were added. After 1.5 h incubation, nonradioactive methionine and leucine were added to final concentrations of 0.4 µM and 0.5 µM, respectively, to chase the radioactive amino acids. Samples were incubated for another 1.5 h to stabilize pulses in bacterioplankton cells before taking subsamples (120 mL fixed with 1% (w/v) paraformaldehyde at 3 h and 9 h) for the measurement of protist tracer uptake rates. Subsequently, different phytoplankton populations (i.e. bacterioplankton, small (approx. 2 µm) and large (approx. 3.1 µm) plastidic protists as well as aplastidic protists) were sorted according to light-scattering properties, relative concentration of SYBR Green I stain per particle and chlorophyll content using a FACSort flow cytometer (Becton Dickinson, UK). Radio-assaying of samples was carried out using an ultra-low-level liquid scintillation counter (1220 Quantulus, Wallac).

Temporal coverage 14 October 2010 - 21 November 2010
Spatial coverage Atlantic Ocean
File format Delimited
Language English
Discovery metadata record Link to the related European Directory of Marine Environmental Data (EDMED) record
Publisher British Oceanographic Data Centre - Natural Environment Research Council, UK
Publication date 16 April 2014
Digital Object Identifier (DOI) doi:10.5285/f7021d34-0290-70fc-e044-000b5de50f38
Short DOI doi:10/sc6
Citation text Hartmann M.; Grob C.; Zubkov M.V. (2014). Influence of light on bacterivory of the smallest eukaryotes on cruise AMT20 (JC053). British Oceanographic Data Centre - Natural Environment Research Council, UK. doi:10/sc6.

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