Metadata Report for BODC Series Reference Number 1921177

• Metadata Summary

• Problem Reports

• Data Access Policy

• Data Policy

• Narrative Documents

• Project Information

• Data Activity or Cruise Information

• Fixed Station Information

• BODC Quality Flags

• SeaDataNet Quality Flags

Metadata Summary

Problem Reports

No Problem Report Found in the Database

Data Access Policy

COMICS research programme data access conditions - phase 1

These data are currently restricted to the COMICS Community.

Data Policy

COMICS Data Policy - phase 1

These data are currently restricted to the COMICS Community. The restriction timeline is based on the data submission/accession date due to the amount of time taken to work up some of the data. Individual datasets are restricted to the COMICS project participants for two years after their accession date.

Narrative Documents

SEAL Analytical QuAAtro colorimetric autoanalyser

The SEAL QuAAtro high Performance Microflow Analyzer is the latest generation of the original world-class Technicon^TM Segmented Flow Analysis (SFA) systems.

A basic SFA system consists of an autosampler, a peristaltic pump, a chemistry manifold, a detector and data acquisition software. Sample and reagents are pumped continuously through the chemistry manifold. Air bubbles are introduced at regular intervals forming unique reaction segments which are mixed using glass coils. Glass is ideal, as it is inert, stays clean and enables easy visual checks.

In SFA, reactions run to completion and the ratio of sample to reagents in the detector reaches a constant maximum value. This results in ultra-low detection limits and exceptional reproducibility. Variations in reaction time, temperature and sample matrix do not affect the results as they do in other colorimetric techniques, such as flow injection analysis, where the reaction is not brought to completion.

QuAAtro is a microflow SFA system, the internal diameter of all glassware being 1 mm. This reduces reagent consumption and increases throughput, with most methods running at 100 - 120 samples hour. The integrated enclosed manifold and detector are heated to 37 °C. Flow stability is ensured as the optimal bubble frequency for each method is programmed by silent air valves. Automatic start-up, method changeover and shutdown allows true unattended operation and overnight running. QuAAtro checks its own performance, with automatic monitoring of noise, drift, bubble pattern and light energy, before and during a run.

Up to four methods can run at the same time on one console, and there is a special 5-channel version for nutrients in seawater. Two consoles can be combined to give an 8-channel system.

Further details can be found in the manufacturer's specification sheet.

Niskin Bottle

The Niskin bottle is a device used by oceanographers to collect subsurface seawater samples. It is a plastic bottle with caps and rubber seals at each end and is deployed with the caps held open, allowing free-flushing of the bottle as it moves through the water column.

Standard Niskin

The standard version of the bottle includes a plastic-coated metal spring or elastic cord running through the interior of the bottle that joins the two caps, and the caps are held open against the spring by plastic lanyards. When the bottle reaches the desired depth the lanyards are released by a pressure-actuated switch, command signal or messenger weight and the caps are forced shut and sealed, trapping the seawater sample.

Lever Action Niskin

The Lever Action Niskin Bottle differs from the standard version, in that the caps are held open during deployment by externally mounted stainless steel springs rather than an internal spring or cord. Lever Action Niskins are recommended for applications where a completely clear sample chamber is critical or for use in deep cold water.

Clean Sampling

A modified version of the standard Niskin bottle has been developed for clean sampling. This is teflon-coated and uses a latex cord to close the caps rather than a metal spring. The clean version of the Levered Action Niskin bottle is also teflon-coated and uses epoxy covered springs in place of the stainless steel springs. These bottles are specifically designed to minimise metal contamination when sampling trace metals.

Deployment

Bottles may be deployed singly clamped to a wire or in groups of up to 48 on a rosette. Standard bottles and Lever Action bottles have a capacity between 1.7 and 30 L. Reversing thermometers may be attached to a spring-loaded disk that rotates through 180° on bottle closure.

RRS Discovery Cruise DY086 CTD Bottle Nutrient Samples

Originator's Protocol for Data Acquisition and Analysis

Nutrient samples were collected during cruise DY086 between November 12^th 2017 and December 19^th 2017. Samples were obtained from a range of depths using an SBE 911plus Titanium or Stainless Steel CTD unit with 24 10L or 20L Niskin bottles fitted to the rosette sampler respectively. Samples were drawn from the Niskin bottles into 15ml centrifuge tubes after the centrifuge tubes were rinsed three times with water from the Niskin bottle being sampled. Analysis was performed within 24-36 hours after the samples were taken using a QuAAtro 39 segmented flow autoanalyser linked to a XY-2 Sampler, both by SEAL Analytical, using appropriate software also supplied by SEAL.

BODC Data Processing Procedures

The nutrient data were supplied to BODC in an Excel spreadsheet. The data were loaded into BODC's database using established BODC data banking procedures with no changes.

The originator's variables were mapped to appropriate BODC parameter codes as follows:

Any flags on the originator's data were mapped to the appropriate equivalent BODC flags. The samples analysed were never below the calculated detection limit. Although some bottles were marked misfired by the originator, BODC did not apply any additional flags to the data from these bottles as the data may still be accurate.

The titanium CTD was used for casts 1, 4, 7, 15, 19, 24, 26, and 29.

Project Information

Controls over Ocean Mesopelagic Interior Carbon Storage: COMICS

COMICS is a four-year collaborative research project that aims to quantify the flow of carbon in the ocean?s ?twilight? zone in order to accurately model global climate change. This ?twilight? zone is the part of the ocean between 100m and 1000m below the sea surface, where only a small amount of light from the sun can still penetrate.

By investigating carbon dynamics in the ocean interior, COMICS will help to improve predictions of future global climate change.

The COMICS project is led by the National Oceanography Centre and is a collaboration between the British Antarctic Survey and the universities of Queen Mary London, Liverpool, Oxford and Southampton. The project received funding from the Natural Environmental Research Council and runs between April 2017 and 2021.

Data Activity or Cruise Information

Data Activity

BODC Sample Metadata Report for DY086_CTD_CTD012

Please note:the supplied parameters may not have been sampled from all the bottle firings described in the table above. Cross-match the Sample Reference Number above against the SAMPRFNM value in the data file to identify the relevant metadata.

Related Data Activity activities are detailed in Appendix 1

Cruise

Complete Cruise Metadata Report is available here

Fixed Station Information

No Fixed Station Information held for the Series

BODC Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

SeaDataNet Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

Appendix 1: DY086_CTD_CTD012

Related series for this Data Activity are presented in the table below. Further information can be found by following the appropriate links.

If you are interested in these series, please be aware we offer a multiple file download service. Should your credentials be insufficient for automatic download, the service also offers a referral to our Enquiries Officer who may be able to negotiate access.