Metadata Report for BODC Series Reference Number 2150712
Metadata Summary
Problem Reports
Data Access Policy
Narrative Documents
Project Information
Data Activity or Cruise Information
Fixed Station Information
BODC Quality Flags
SeaDataNet Quality Flags
Metadata Summary
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Problem Reports
No Problem Report Found in the Database
Data Access Policy
Open Data supplied by Natural Environment Research Council (NERC)
You must always use the following attribution statement to acknowledge the source of the information: "Contains data supplied by Natural Environment Research Council."
Narrative Documents
Seapoint Chlorophyll Fluorometer
The Seapoint Chlorophyll Fluorometer (SCF) is a low power instrument for in situ measurements of chlorophyll a. The SCF uses modulated blue LED lamps and a blue excitation filter to excite chlorophyll a. The fluorescent light emitted by the chlorophyll a passes through a red emission filter and is detected by a silicon photodiode. The low level signal is then processed using synchronous demodulation circuitry which generates an output voltage proportional to chlorophyll a concentration. The SCF may be operated with or without a pump.
Sensor specifications, current at August 2006, are given in the table below. More information can be found at the manufacturer's web site.
Sensor Specifications
Power requirements | 8 - 20 VDC, 15 mA avg., 27 mA pk. |
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Output | 0 - 5.0 VDC |
Output Time Constant | 0.1 sec. |
Power-up transient period | < 1 sec. |
Excitation Wavelength | 470 nm CWL, 30 nm FWHM |
Emission Wavelength | 685 nm CWL, 30 nm FWHM |
Sensing Volume | 340 mm3 |
Minimum Detectable Level | 0.02 µg l-1 |
Gain | Sensitivity, V µg-1 l-1 | Range, µg l-1 | |
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Sensitivity/Range | 30x 10x 3x 1x | 1.0 0.33 0.1 0.033 | 5 15 50 150 |
Turner Designs Trilogy Fluorometer
The Trilogy Laboratory Fluorometer is a compact laboratory instrument for making fluorescence, absorbance and turbidity measurements using the appropriate snap-in Application Module.
The following snap-in application modules are available:
Application | Minimum Detection Limit | Linear Range | Comments | |
---|---|---|---|---|
Ammonium | 0.05µmol | 0-50µmol | ||
CDOM/FDOM | 0.1 ppb | 0 - 1000 ppb | Quinine sulphate | |
Chlorophyll-a extracted (acidification) | 0.025 µg l-1 | 0-300 µg l-1 | ||
Chlorophyll-a extracted (non-acidification) | 0.025 µg l-1 | 0-300 µg l-1 | ||
Chlorophyll in vivo | 0.025 µg l-1 | 0-300 µg l-1 | ||
Fluorescein dye standard range | 0.01 ppb | 0-200 ppb | ||
Fluorescein dye extended range | 0.75 ppb | 0-8000 ppb | Minicell adapter P/N 8000-936 and P/N 7000-950 required | |
Histamine | 0.001 ppm | 0-100 ppm | ||
Histamine (PTSA) | 0.5 ppm | 0-2,000 ppm | ||
Nitrate (absorbance) | 0.04 mg l-1 | 0 - 14 mg l-1 | ||
Crude Oil | 0.2 ppb | 0 - 2,000 ppb | Quinine sulphate | |
Refined Oil | 0.25 ppb | 0 - 6,000 ppb Napthalene | 1,5 Naphthalene disulfonic disodium salt | |
Optical Brighteners | 1 ppb | 0 - 10,000 ppb | Quinine sulphate | |
Phosphate (absorbance) | 1 µg l-1 | 0 - 930 µg l-1 | ||
Phycocyanin (freshwater) | 150 cells ml-1 | 0 - 150,000 cells ml-1 | ||
Phycoerythrin (marine) | 150 cells ml-1 | 0 - 150,000 cells ml-1 | ||
Pyrene tetra sulfonic acid (PTSA) | 0.1 ppb | >10,000 ppb | ||
Rhodamine WT | 0.01 ppb | 0 - 500 ppb | ||
Silicate (absorbance) | 3 µg l-1 | 0 - 3000 µg l-1 | ||
Turbidity | 0.05 NTU | 0 - 1000 NTU |
For extracted chlorophyll measurements using EPA 445, Trilogy automatically calculates the concentration using the filtered and solvent volumes. The turbidity modules use an IRLED with a wavelength of 860nm to meet ISO 7027 standards for turbidity water quality measurements.
Specifications
Power | 100 to 240VAC Universal Power Supply, Output 12VDC 0.84A Max |
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Operating Temperature | 15-40 °C |
Size | 32.82 cm depth, 26.52 cm width, 21.39 height |
Weight | 3.65 kg |
Readout | Direct concentration (µg l-1, ppb etc.) |
Light source and detector | Light emitting diode and photodiode |
Data output | 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud |
PC operating system | Windows 98 or later |
Further details can be found in the manufacturer's specification sheet.
Non-toxic (underway) sea water supply
A source of uncontaminated near-surface (commonly 3 to 7 m) seawater pumped continuously to shipboard laboratories on research vessels. There is typically a temperature sensor near the intake (known as the hull temperature) to provide measurements that are as close as possible to the ambient water temperature. The flow from the supply is typically directed through continuously logged sensors such as a thermosalinograph and a fluorometer. Water samples are often collected from the non-toxic supply. The system is also referred to as the underway supply.
LOCATE Cruise CEnd 18/16 Underway Discrete Samples
Originator's Protocol for Data Acquisition and Analysis
A total of 148 water samples were taken during the LOCATE cruise CEnd 18/16 from the ship's non-toxic supply roughly every two-three hours. The samples were passed through the ship's Ferrybox system, which took measurements of temperature, chlorophyll a, salinity, and photosynthetically available radiation. Samples were measured by the Centre for Environment Fishers and Aquaculture Science (CEFAS), National Oceanography Centre (NOC), Plymouth Marine Laboratory (PML), Centre for Ecology and Hydrography (CEH), and Thermodynamic Equation of Seawater - 2010 (TEOS-10), although the TEOS-10 data was not processed. There was an issue with the navigation channel of the Ferrybox, therefore the location of these data are approximate positions calculated by the data originator.
Chlorophyll-a
Chlorophyll-a concentrations were determined fluorometrically from 250ml water samples. The water samples were then filtered through 25mm Whatman GF/F filters with a nominal pore size ~0.7mm and the pigments were then extracted in 6ml of 90% acetone at 4°C over an 18-20 hour period. Pigment extracts were measured using a Turner Designs Trilogy fluorometer following Welschmeyer (1994) and calibrated against a pure chlorophyll-a standard. The CEFAS Ferrybox chlorophyll was calibrated against the NOC chlorophyll using the equation: y=4.8106x-0.083.
Inorganic Nutrients - National Oceanography Centre
Water samples were collected, filtered through 0.2µm filters and frozen in 15 ml Falcon tubes for later analysis. Samples were analysed using a Seal Quaatro autoanalyser.
Inorganic Nutrients - Plymouth Marine Laboratory
Water samples were collected, filtered through 0.2µm filters, and frozen in 60 ml HDPE Nalgene bottles for later analysis at PML using standard procedures on a Technicon segmented flow colorimetric autoanalyser following standard procedures (e.g. Woodward and Rees (2001)).
Dissolved Organic Nitrogen and Dissolved Organic Phosphorous
Water samples were collected, filtered through 0.2µm filters for samples 1-21 and frozen in 125 ml HDPE bottles for later analysis. The inline filtration system did not work properly, and the filter frequently leaked, so there is low confidence in the filtering and the water samples were not filtered from sample 22 onward. During analysis, samples were warmed to room temperature, defrosted, and throughly mixed. Concentrations of dissolved organic nitrogen (DON) and dissolved organic phosphorous (DOP) were obtained by UV-oxidation of duplicate 10 ml aliquots using Methrohm 205 UV digestors. Samples were irradiated for 2 hours before analysis for total inorganic nitrate and phosphorous concentrations using a Seal Quaatro autoanalyser and nutrient certified reference matereials (CRM's) (from Kanso, Japan) for reference and correction following GO-SHIP recommended protocols. Concentrations of DON and DOP were obtained by difference (i.e. DON = TDN - NO3+NO2; DOP = TDP - PO4).
Dissolved Organic Carbon
Dissolved organic carbon (DOC) samples were analysed at CEH in Lancaster. Water samples were collected, filtered through 0.2µm filters for samples 1-21 and frozen in 125 ml HDPE bottles for later analysis. The inline filtration system did not work properly, and the filter frequently leaked, so there is low confidence in the filtering and the water samples were not filtered from sample 22 onward. The samples were acidified with 3M HCl and sparged with oxygen, then analysed on a Skalar Formacs analyser. Inorganic carbon is converted to CO2 and removed. The remaining non-purgeable organic carbon (NPOC) can be regarded as DOC for waters where there are no volatile organic carbon compounds present.
Particulate Organic Nitrogen and Particulate Organic Carbon
Samples were analysed using the Southampton University EA-IRMS system. Samples were acid fumed prior to analysis to remove particulate inorganic carbon, and underwent elemental analysis before being run through the mass spectrometer. The samples were run on two different machines:
- Vario PYRO cube elemental analyser coupled with vision isotope ratio mass spectrometer in CNS and CN modes
- Vario ISOTOPE select elemental analyser coupled with Isoprime 100 Isotope ratio mass spectrometer in CN mode
Particulate Organic Phosphorus
Samples were analysed using the chemical digestion method of Raimbault et al (1999) and run through an autoanalyser.
Salinity
Salinity samples were analysed back at NOC using a Guildline 8410 Portasal salinometer.
References Cited
Painter, S. C. et al. (2018) Terrestrial dissolved organic matter distribution in the North Sea, Science of The Total Environment, 630, 630-647.
Raimbault, P. et al. (1999) Simultaneous determination of particulate organic carbon, nitrogen and phosphorus collected on filters, using a semi-automatic wet-oxidation method. Marine Ecology Progress Series, 180, 289-295.
Welschmeyer, N. A. (1994) Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography, 39(8), 1985-1992.
Woodward, E. M. S. and Rees, A. P. (2001) Nutrient distributions in an anticyclonic eddy in the northeast Atlantic Ocean, with reference to nanomolar ammonium concentrations. Deep Sea Research Part II, 48, 775-793.
BODC Data Processing Procedures
The data were supplied to BODC in one Excel file. The data from different organisations were processed together by BODC as they originated from the same water samples, but should be considered separate datasets. The data from PML should be considered primary and the NOC data secondary.
The originator variables were mapped to appropriate BODC parameter codes as follows:
Originator's Variable | Originator's Units | BODC Parameter Code | BODC Units | Comment |
---|---|---|---|---|
PRT-100-SST | °C | PSSTTS01 | °C | CEFAS Ferrybox |
Chl-a | RFU | CPHLPR01 | mg/m3 | calibration y=4.8106x-0.083 to mg/m3 CEFAS Ferrybox |
Salinity | psu | PSALTC01 | Dimensionless | CEFAS Ferrybox |
SST | °C | TEMPPR01 | °C | CEFAS Ferrybox |
PAR | µE/m2/s | PARERXUD | µE/m2/s | CEFAS Ferrybox |
Fluorometric Chl-a | µg/l | CPHLFLP1 | mg/m3 | µg/l=mg/m3 NOC |
Bottle Salinity | psu | PSALBSTX | Dimensionless | NOC |
TDN | µmol/l | CONIWA13 | µmol/l | NOC |
TDP | µmol/l | COPHWA14 | µmol/l | NOC |
NO3 | µmol/l | NTRZAAD5 | µmol/l | NOC |
Si | µmol/l | SLCAAAD5 | µmol/l | NOC |
NO2 | µmol/l | NTRIAAD5 | µmol/l | NOC |
PO4 | µmol/l | PHOSAAD5 | µmol/l | NOC |
DON | µmol/l | MDMAP009 | µmol/l | NOC |
DOP | µmol/l | ORGPDSZZ | µmol/l | NOC |
NO2_pml | µmol/l | NTRIAAD5 | µmol/l | PML |
NO3_2_pml | µmol/l | NTRZAAD5 | µmol/l | PML |
NH4_pml | µmol/l | AMONAAD5 | µmol/l | PML |
Si_pml | µmol/l | SLCAAAD5 | µmol/l | PML |
PO4_pml | µmol/l | PHOSAAD5 | µmol/l | PML |
DOC | µmol/l | CORGZZZX | µmol/l | CEH |
POC | µmol/l | CORGCAPZ | µmol/l | University of Southampton |
PON | µmol/l | NTOTCNPZ | µmol/l | University of Southampton |
POP | µmol/l | OPHSVLPT | µmol/l | University of Southampton |
Note to User
This dataset is made freely available to allow reuse of the data and in addition to the source publication being recognised we expect and encourage that LOCATE project datasets are acknowledged in all future publications via inclusion of the LOCATE grant code in the acknowledgements. Further analysis of this data can be found in Painter et al (2018).
Data citation
If using this data, please add the following citation. "Data used in this study were supplied by the Land Ocean Carbon Transfer (LOCATE) project, a study supported by Natural Environment Research Council grant NE/N018087/1".
Other data
BODC also holds data for normalised fluorescent intensities (in Raman Units) of the 3 organic matter compounds using PARAFAC modelling analysis. However, these remain in the original format and were not ingested due to missing underlying data to support the results. There are also data derived using TEOS-10, Conservative Temperature and Absolute Salinity, that were not ingested as they are derived parameters and can be calculated from the ingested data. All of these are available upon request.
Project Information
Land Ocean CArbon TransfEr: LOCATE
LOCATE is a collaboration between the National Oceanography Centre (NOC), the Centre for Ecology and Hydrology (CEH), the British Geological Survey (BGS) and the Plymouth Marine Laboratory (PML).
LOCATE integrates knowledge and skills across NERC terrestrial, freshwater and marine research centres to transform the UK?s ability to measure, model and predict land-ocean tOM fluxes in a changing environment. It allows next-generation biogeochemical models to explore how climate change and land management decisions influence the global carbon cycle. The project has three main objectives:
- To quantify the fate of terrigeneous organic matter from soils to the ocean, with particular focus on estuaries and coastal waters.
- To quantify and understand the loss processes in estuarine environments.
- To build a new model of terrigeneous organic matter cycling valid in marine and fresh waters, and use it to predict the future evolution of the land ocean carbon flux.
LOCATE is funded by the Natural Environment Research Council (NERC) from 2016 until 2021.
Data Activity or Cruise Information
Cruise
Cruise Name | 18/16 |
Departure Date | 2016-08-08 |
Arrival Date | 2016-09-07 |
Principal Scientist(s) | Benjamin Hatton (Centre for Environment, Fisheries and Aquaculture Science Lowestoft Laboratory) |
Ship | RV Cefas Endeavour |
Complete Cruise Metadata Report is available here
Fixed Station Information
No Fixed Station Information held for the Series
BODC Quality Control Flags
The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:
Flag | Description |
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Blank | Unqualified |
< | Below detection limit |
> | In excess of quoted value |
A | Taxonomic flag for affinis (aff.) |
B | Beginning of CTD Down/Up Cast |
C | Taxonomic flag for confer (cf.) |
D | Thermometric depth |
E | End of CTD Down/Up Cast |
G | Non-taxonomic biological characteristic uncertainty |
H | Extrapolated value |
I | Taxonomic flag for single species (sp.) |
K | Improbable value - unknown quality control source |
L | Improbable value - originator's quality control |
M | Improbable value - BODC quality control |
N | Null value |
O | Improbable value - user quality control |
P | Trace/calm |
Q | Indeterminate |
R | Replacement value |
S | Estimated value |
T | Interpolated value |
U | Uncalibrated |
W | Control value |
X | Excessive difference |
SeaDataNet Quality Control Flags
The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:
Flag | Description |
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0 | no quality control |
1 | good value |
2 | probably good value |
3 | probably bad value |
4 | bad value |
5 | changed value |
6 | value below detection |
7 | value in excess |
8 | interpolated value |
9 | missing value |
A | value phenomenon uncertain |
B | nominal value |
Q | value below limit of quantification |