Cruise inventory

RRS Charles Darwin CD132 (AMBITION)

Cruise summary report

Cruise Info. 
Ship name (ship code)RRS Charles Darwin (74AB)
Cruise identifierCD132 (AMBITION)
Cruise period2001-08-30 — 2001-09-29
StatusCompleted
Port of departureVictoria, Seychelles
Port of returnMuscat, Oman
PurposeResearch
ObjectivesThe objectives of the five participating M&FMB projects were:
1) To collect material (preserved filtrates and frozen samples) for DNA extraction and analysis on return to Cardiff, particularly in more eutrophic waters [Fry & Weightman (Cardiff): Isolation and characterisation of unculturable? Heterotrophic marine bacteria].
2) To sample the upper-ocean for methyl bromide (MeBr) and isolation of MeBr-utilising bacteria. Carry out onboard experiments using gas chromatographs, liquid nitrogen, gas bottles and incubators. [Murrell & McDonald (Warwick), Nightingale (PML): Methyl bromide metabolism in the marine environment ]
3) To sample the horizontal and vertical distributions of picoeukaryotes in relation to environmental gradients. Also investigate temporal responses (diel changes in situ and via deck incubations) and carry out DNA and RNA analyses. [Scanlan (Warwick): In situ community structure of marine photosynthetic picoeukaryotes ]
4) To generate a range of size-fractionated samples for analysis of nif gene diversity, together with information on environmental conditions (to determine factors affecting gene expression). [Wyman (Stirling) & O?Donnell (Newcastle): Nitrogen fixing microbes in the sea - a molecular approach linking phenotype to 16S rRNA phylotype]
5) To characterise bacterioplankton community and to investigate their links to biogeochemical functions. [Zubkov & Burkill (PML): Bacterial biodiversity; dominance, competitiveness and functionality within communities]
Chief scientistPeter H Burkill (Plymouth Marine Laboratory)
ProjectMarine and Freshwater Microbial Biodiversity (M&FMB)
Cruise reportRRS Charles Darwin CD132 (AMBITION) cruise report cd132.pdf — access key 'C' (3.72 MB) 
Ocean/sea areas 
GeneralIndian Ocean
Arabian Sea
Track chartsRRS Charles Darwin CD132 (AMBITION) cruise track — cd132trk.pdf (00 MB) 
Measurements 
Physical oceanography 
CTD stationsDescription: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples
Biology and fisheries 
Phytoplankton pigs (eg chlorophyll, fluorescence)Description: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples
PhytoplanktonDescription: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples
Data held at BODC 
Series/Profiles 
69  Bottle station
Quality control flags
Ammonium and ammonia concentration parameters in water bodies
Chlorophyll pigment concentrations in water bodies
Silicate concentration parameters in the water column
Vertical spatial coordinates
Carotenoid and flavenoid pigment concentrations in water bodies
Nitrate concentration parameters in the water column
Reference numbers
Nitrite concentration parameters in the water column
Phosphate concentration parameters in the water column
Quality control flags
Ammonium and ammonia concentration parameters in water bodies
Chlorophyll pigment concentrations in water bodies
Silicate concentration parameters in the water column
Vertical spatial coordinates
Carotenoid and flavenoid pigment concentrations in water bodies
Nitrate concentration parameters in the water column
Reference numbers
Nitrite concentration parameters in the water column
Phosphate concentration parameters in the water column

BODC idDate/TimeLocationDoc.
12805262001-09-10 08:35 7° 36' 1" N  67° 0' 15" E  View metadata report for BODC series reference number 1280526
12805382001-09-11 00:26 7° 36' 6" N  67° 0' 16" E  View metadata report for BODC series reference number 1280538
12805512001-09-11 05:55 7° 36' 3" N  67° 2' 29" E  View metadata report for BODC series reference number 1280551
12805632001-09-12 08:19 11° 23' 39" N  66° 59' 47" E  View metadata report for BODC series reference number 1280563
12805752001-09-13 00:28 11° 23' 44" N  66° 59' 50" E  View metadata report for BODC series reference number 1280575
12805872001-09-13 05:33 11° 22' 41" N  66° 59' 39" E  View metadata report for BODC series reference number 1280587
12805992001-09-14 07:47 15° 11' 54" N  66° 59' 56" E  View metadata report for BODC series reference number 1280599
12806062001-09-15 00:29 15° 11' 56" N  67° 0' 1" E  View metadata report for BODC series reference number 1280606
12806182001-09-16 08:31 18° 59' 58" N  67° 0' 6" E  View metadata report for BODC series reference number 1280618
12806312001-09-16 14:30 19° 0' 3" N  67° 0' 2" E  View metadata report for BODC series reference number 1280631
12806432001-09-16 20:32 19° 0' 1" N  67° 0' 11" E  View metadata report for BODC series reference number 1280643
12806552001-09-17 02:21 19° 0' 4" N  67° 0' 0" E  View metadata report for BODC series reference number 1280655
12806672001-09-18 00:29 18° 59' 51" N  66° 59' 55" E  View metadata report for BODC series reference number 1280667
12806792001-09-19 08:28 20° 54' 49" N  63° 39' 56" E  View metadata report for BODC series reference number 1280679
12806802001-09-20 00:29 20° 54' 48" N  63° 39' 54" E  View metadata report for BODC series reference number 1280680
12806922001-09-22 08:32 23° 33' 11" N  59° 53' 50" E  View metadata report for BODC series reference number 1280692
12807112001-09-23 00:31 23° 33' 16" N  59° 54' 1" E  View metadata report for BODC series reference number 1280711
12807232001-09-24 08:30 24° 19' 55" N  58° 10' 3" E  View metadata report for BODC series reference number 1280723
12807352001-09-25 00:31 24° 19' 55" N  58° 10' 3" E  View metadata report for BODC series reference number 1280735
12807472001-09-26 08:28 26° 0' 5" N  56° 34' 56" E  View metadata report for BODC series reference number 1280747
12807592001-09-27 00:24 26° 0' 7" N  56° 35' 3" E  View metadata report for BODC series reference number 1280759
12803542001-09-01 10:40 2° 26' 41" S  59° 22' 15" E  View metadata report for BODC series reference number 1280354
12803662001-09-03 01:33 0° 54' 41" S  64° 8' 30" E  View metadata report for BODC series reference number 1280366
12803782001-09-03 07:02 0° 53' 24" S  64° 8' 12" E  View metadata report for BODC series reference number 1280378
12803912001-09-04 03:31 0° 54' 40" S  64° 8' 19" E  View metadata report for BODC series reference number 1280391
12804092001-09-05 00:26 0° 0' 9" S  66° 59' 55" E  View metadata report for BODC series reference number 1280409
12804102001-09-05 06:03 0° 1' 13" S  66° 59' 30" E  View metadata report for BODC series reference number 1280410
12804222001-09-06 02:29 0° 1' 13" N  67° 1' 22" E  View metadata report for BODC series reference number 1280422
12804342001-09-07 08:41 3° 48' 1" N  67° 0' 2" E  View metadata report for BODC series reference number 1280434
12804462001-09-07 14:33 3° 47' 57" N  67° 0' 5" E  View metadata report for BODC series reference number 1280446
12804582001-09-07 17:29 3° 47' 57" N  67° 0' 14" E  View metadata report for BODC series reference number 1280458
12804712001-09-07 20:30 3° 47' 50" N  67° 0' 0" E  View metadata report for BODC series reference number 1280471
12804832001-09-08 02:25 3° 47' 58" N  67° 0' 3" E  View metadata report for BODC series reference number 1280483
12804952001-09-08 08:28 3° 47' 33" N  67° 0' 4" E  View metadata report for BODC series reference number 1280495
12805022001-09-09 00:30 3° 47' 26" N  67° 0' 1" E  View metadata report for BODC series reference number 1280502
12805142001-09-09 06:31 3° 45' 57" N  66° 59' 7" E  View metadata report for BODC series reference number 1280514
16550662001-09-01 10:40 2° 26' 41" S  59° 22' 15" E  View metadata report for BODC series reference number 1655066
16550782001-09-03 01:33 0° 54' 41" S  64° 8' 30" E  View metadata report for BODC series reference number 1655078
16550912001-09-03 09:02 0° 54' 53" S  64° 8' 21" E  View metadata report for BODC series reference number 1655091
16551092001-09-04 03:31 0° 54' 40" S  64° 8' 19" E  View metadata report for BODC series reference number 1655109
16551102001-09-05 00:26 0° 0' 9" S  66° 59' 55" E  View metadata report for BODC series reference number 1655110
16551222001-09-05 08:41 0° 0' 14" S  67° 0' 4" E  View metadata report for BODC series reference number 1655122
16551342001-09-06 02:29 0° 1' 13" N  67° 1' 22" E  View metadata report for BODC series reference number 1655134
16551462001-09-07 08:41 3° 48' 1" N  67° 0' 2" E  View metadata report for BODC series reference number 1655146
16551582001-09-07 14:33 3° 47' 57" N  67° 0' 5" E  View metadata report for BODC series reference number 1655158
16551712001-09-07 20:30 3° 47' 50" N  67° 0' 0" E  View metadata report for BODC series reference number 1655171
16551832001-09-08 02:25 3° 47' 58" N  67° 0' 3" E  View metadata report for BODC series reference number 1655183
16551952001-09-08 08:28 3° 47' 33" N  67° 0' 4" E  View metadata report for BODC series reference number 1655195
16552022001-09-09 00:30 3° 47' 26" N  67° 0' 1" E  View metadata report for BODC series reference number 1655202
16552142001-09-10 08:35 7° 36' 1" N  67° 0' 15" E  View metadata report for BODC series reference number 1655214
16552262001-09-11 00:26 7° 36' 6" N  67° 0' 16" E  View metadata report for BODC series reference number 1655226
16552382001-09-12 08:19 11° 23' 39" N  66° 59' 47" E  View metadata report for BODC series reference number 1655238
16552512001-09-13 00:28 11° 23' 44" N  66° 59' 50" E  View metadata report for BODC series reference number 1655251
16552632001-09-14 07:47 15° 11' 54" N  66° 59' 56" E  View metadata report for BODC series reference number 1655263
16552752001-09-15 00:29 15° 11' 56" N  67° 0' 1" E  View metadata report for BODC series reference number 1655275
16552872001-09-16 08:31 18° 59' 58" N  67° 0' 6" E  View metadata report for BODC series reference number 1655287
16552992001-09-16 14:30 19° 0' 3" N  67° 0' 2" E  View metadata report for BODC series reference number 1655299
16553062001-09-16 20:32 19° 0' 1" N  67° 0' 11" E  View metadata report for BODC series reference number 1655306
16553182001-09-17 02:21 19° 0' 4" N  67° 0' 0" E  View metadata report for BODC series reference number 1655318
16553312001-09-17 08:25 18° 59' 58" N  66° 59' 58" E  View metadata report for BODC series reference number 1655331
16553432001-09-18 00:29 18° 59' 51" N  66° 59' 55" E  View metadata report for BODC series reference number 1655343
16553552001-09-19 08:28 20° 54' 49" N  63° 39' 56" E  View metadata report for BODC series reference number 1655355
16553672001-09-20 00:29 20° 54' 48" N  63° 39' 54" E  View metadata report for BODC series reference number 1655367
16553792001-09-22 08:32 23° 33' 11" N  59° 53' 50" E  View metadata report for BODC series reference number 1655379
16553802001-09-23 00:31 23° 33' 16" N  59° 54' 1" E  View metadata report for BODC series reference number 1655380
16553922001-09-24 08:30 24° 19' 55" N  58° 10' 3" E  View metadata report for BODC series reference number 1655392
16554112001-09-25 00:31 24° 19' 55" N  58° 10' 3" E  View metadata report for BODC series reference number 1655411
16554232001-09-26 08:28 26° 0' 5" N  56° 34' 56" E  View metadata report for BODC series reference number 1655423
16554352001-09-27 00:24 26° 0' 7" N  56° 35' 3" E  View metadata report for BODC series reference number 1655435
Discrete samples 
65  CTD frame plus rosette samplerAmmonium and ammonia concentration parameters in water bodies
Bacteria taxonomic abundance in water bodies
Phytoplankton taxonomic biomass in water bodies
Phytoplankton generic biomass in water bodies
Particulate total and organic nitrogen concentrations in the water column
Zooplankton taxonomy-related abundance per unit volume of the water column
Molecular biology parameters
Density of the water column
Transmittance and attenuance of the water column
Bacteria generic abundance in water bodies
Microzooplankton taxonomy-related biomass expressed as carbon per unit volume of the water column
Visible waveband radiance and irradiance measurements in the water column
Zooplankton non taxonomy-related biomass expressed as carbon per unit volume of the water column
Primary production in the water column
Chlorophyll pigment concentrations in water bodies
Dissolved organic carbon uptake and production in the water column
Phytoplankton taxonomic abundance in water bodies
Metadata parameters
Bacterial production in the water column
Silicate concentration parameters in the water column
Salinity of the water column
Concentration of suspended particulate material in the water column
Zooplankton non taxonomy-related abundance per unit volume of the water column
Bacteria taxonomy-related biomass expressed as carbon per unit volume of the water column
Carotenoid and flavenoid pigment concentrations in water bodies
Nitrate concentration parameters in the water column
Temperature of the water column
Phytoplankton generic abundance in water bodies
Regenerated production in water bodies
Dissolved oxygen parameters in the water column
Microzooplankton taxonomic abundance in water bodies
Nitrite concentration parameters in the water column
New production in water bodies
Phosphate concentration parameters in the water column
Zooplankton taxonomy-related biomass expressed as carbon per unit volume of the water column
58  Moving Vessel Profiler instrumentDensity of the water column
Chlorophyll pigment concentrations in water bodies
Sound velocity and travel time in the water column
Raw fluorometer output
Salinity of the water column
Temperature of the water column
98  Sample taken from ship's non-toxic supplyChlorophyll pigment concentrations in water bodies
Salinity of the water column
Temperature of the water column
1  Surface water sample collected by a bucket on a ropePhytoplankton taxonomic abundance in water bodies
Phytoplankton generic abundance in water bodies