RRS Charles Darwin CD132 (AMBITION)

Cruise summary report

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Cruise Info.

Ship name (ship code)

RRS Charles Darwin (74AB)

Cruise identifier

CD132 (AMBITION)

Cruise period

2001-08-30 — 2001-09-29

Status

Completed

Port of departure

Victoria, Seychelles

Port of return

Muscat, Oman

Purpose

Research

Objectives

The objectives of the five participating M&FMB projects were:
1) To collect material (preserved filtrates and frozen samples) for DNA extraction and analysis on return to Cardiff, particularly in more eutrophic waters [Fry & Weightman (Cardiff): Isolation and characterisation of unculturable? Heterotrophic marine bacteria].
2) To sample the upper-ocean for methyl bromide (MeBr) and isolation of MeBr-utilising bacteria. Carry out onboard experiments using gas chromatographs, liquid nitrogen, gas bottles and incubators. [Murrell & McDonald (Warwick), Nightingale (PML): Methyl bromide metabolism in the marine environment ]
3) To sample the horizontal and vertical distributions of picoeukaryotes in relation to environmental gradients. Also investigate temporal responses (diel changes in situ and via deck incubations) and carry out DNA and RNA analyses. [Scanlan (Warwick): In situ community structure of marine photosynthetic picoeukaryotes ]
4) To generate a range of size-fractionated samples for analysis of nif gene diversity, together with information on environmental conditions (to determine factors affecting gene expression). [Wyman (Stirling) & O?Donnell (Newcastle): Nitrogen fixing microbes in the sea - a molecular approach linking phenotype to 16S rRNA phylotype]
5) To characterise bacterioplankton community and to investigate their links to biogeochemical functions. [Zubkov & Burkill (PML): Bacterial biodiversity; dominance, competitiveness and functionality within communities]

Chief scientist

Peter H Burkill (Plymouth Marine Laboratory)

Project

Marine and Freshwater Microbial Biodiversity (M&FMB)

Cruise report

(3.72 MB)

Ocean/sea areas

General

Indian Ocean
Arabian Sea

Track charts

(0.47 MB)

Measurements

Physical oceanography

CTD stations

Description: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples

Biology and fisheries

Phytoplankton pigs (eg chlorophyll, fluorescence)

Description: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples

Phytoplankton

Description: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples

Data held at BODC

Get data

Series/Profiles

168 Bottle station

Reference numbers
Nitrite concentration parameters in the water column
Carotenoid and flavenoid pigment concentrations in water bodies
Particulate total and organic nitrogen concentrations in the water column
Vertical spatial coordinates
Phosphate concentration parameters in the water column
Phytoplankton taxonomic abundance in water bodies
Salinity of the water column
Silicate concentration parameters in the water column
Bacteria generic abundance in water bodies
Ammonium and ammonia concentration parameters in water bodies
Phytoplankton generic abundance in water bodies
Nitrate concentration parameters in the water column
Quality control flags
Chlorophyll pigment concentrations in water bodies

[Show series]

[Hide series]

BODC id	Date/Time	Location
1280354	2001-09-01 10:40	2° 26' 41" S 59° 22' 15" E
1280538	2001-09-11 00:26	7° 36' 6" N 67° 0' 16" E
1280618	2001-09-16 08:31	18° 59' 58" N 67° 0' 6" E
1280643	2001-09-16 20:32	19° 0' 1" N 67° 0' 11" E
1280667	2001-09-18 00:29	18° 59' 51" N 66° 59' 55" E
1280747	2001-09-26 08:28	26° 0' 5" N 56° 34' 56" E
1280759	2001-09-27 00:24	26° 0' 7" N 56° 35' 3" E
1655091	2001-09-03 09:02	0° 54' 53" S 64° 8' 21" E
1655158	2001-09-07 14:33	3° 47' 57" N 67° 0' 5" E
1655183	2001-09-08 02:25	3° 47' 58" N 67° 0' 3" E
1655318	2001-09-17 02:21	19° 0' 4" N 67° 0' 0" E
1655343	2001-09-18 00:29	18° 59' 51" N 66° 59' 55" E
2272770	2001-09-04 03:30:30	0° 54' 40" S 64° 8' 19" E
2272954	2001-09-18 00:28:30	18° 59' 51" N 66° 59' 55" E
2273017	2001-09-24 08:29:30	24° 19' 55" N 58° 10' 3" E
2273078	2001-09-11 00:26	7° 36' 6" N 67° 0' 16" E
2273158	2001-09-01 10:40	2° 26' 41" S 59° 22' 15" E
2273238	2001-09-07 14:33	3° 47' 57" N 67° 0' 5" E
2273355	2001-09-13 00:27:30	11° 23' 44" N 66° 59' 50" E
2273367	2001-09-13 05:33	11° 22' 41" N 66° 59' 39" E
2273392	2001-09-16 08:30:30	18° 59' 58" N 67° 0' 6" E
2273411	2001-09-16 14:30	19° 0' 3" N 67° 0' 2" E
2273447	2001-09-18 00:28:30	18° 59' 51" N 66° 59' 55" E
2273503	2001-09-23 00:31	23° 33' 16" N 59° 54' 1" E
2273527	2001-09-24 08:29:30	24° 19' 55" N 58° 10' 3" E
1280446	2001-09-07 14:33	3° 47' 57" N 67° 0' 5" E
1280495	2001-09-08 08:28	3° 47' 33" N 67° 0' 4" E
1280711	2001-09-23 00:31	23° 33' 16" N 59° 54' 1" E
1655109	2001-09-04 03:31	0° 54' 40" S 64° 8' 19" E
1655226	2001-09-11 00:26	7° 36' 6" N 67° 0' 16" E
1655380	2001-09-23 00:31	23° 33' 16" N 59° 54' 1" E
1655392	2001-09-24 08:30	24° 19' 55" N 58° 10' 3" E
2272769	2001-09-03 01:33	0° 54' 41" S 64° 8' 30" E
2272782	2001-09-05 00:26	0° 0' 9" S 66° 59' 55" E
2272850	2001-09-10 08:34:30	7° 36' 1" N 67° 0' 15" E
2272862	2001-09-11 00:26	7° 36' 6" N 67° 0' 16" E
2272874	2001-09-12 08:18:30	11° 23' 39" N 66° 59' 47" E
2272886	2001-09-13 00:27:30	11° 23' 44" N 66° 59' 50" E
2272930	2001-09-16 20:31:30	19° 0' 1" N 67° 0' 11" E
2273029	2001-09-25 00:31	24° 19' 55" N 58° 10' 3" E
2273146	2001-09-25 00:31	24° 19' 55" N 58° 10' 3" E
2273343	2001-09-12 08:18:30	11° 23' 39" N 66° 59' 47" E
2273472	2001-09-20 00:29	20° 54' 48" N 63° 39' 54" E
2273484	2001-09-20 06:00:30	20° 55' 9" N 63° 38' 51" E
2273515	2001-09-23 06:44:30	23° 31' 59" N 59° 53' 36" E
2273576	2001-09-03 01:33	0° 54' 41" S 64° 8' 30" E
2273632	2001-09-05 06:02:30	0° 1' 13" S 66° 59' 30" E
1280458	2001-09-07 17:29	3° 47' 57" N 67° 0' 14" E
1280551	2001-09-11 05:55	7° 36' 3" N 67° 2' 29" E
1280575	2001-09-13 00:28	11° 23' 44" N 66° 59' 50" E
1280587	2001-09-13 05:33	11° 22' 41" N 66° 59' 39" E
1655066	2001-09-01 10:40	2° 26' 41" S 59° 22' 15" E
1655331	2001-09-17 08:25	18° 59' 58" N 66° 59' 58" E
2272801	2001-09-07 08:41	3° 48' 1" N 67° 0' 2" E
2272929	2001-09-16 14:30	19° 0' 3" N 67° 0' 2" E
2272942	2001-09-17 02:20:30	19° 0' 4" N 67° 0' 0" E
2272966	2001-09-19 08:28	20° 54' 49" N 63° 39' 56" E
2273066	2001-09-09 00:29:30	3° 47' 26" N 67° 0' 1" E
2273318	2001-09-11 00:26	7° 36' 6" N 67° 0' 16" E
2273331	2001-09-11 05:54:30	7° 36' 3" N 67° 2' 29" E
2273459	2001-09-18 05:31	18° 59' 28" N 67° 0' 15" E
2273620	2001-09-05 00:26	0° 0' 9" S 66° 59' 55" E
2273681	2001-09-09 00:29:30	3° 47' 26" N 67° 0' 1" E
2273761	2001-09-18 05:31	18° 59' 28" N 67° 0' 15" E
2273773	2001-09-19 08:28	20° 54' 49" N 63° 39' 56" E
1280366	2001-09-03 01:33	0° 54' 41" S 64° 8' 30" E
1280391	2001-09-04 03:31	0° 54' 40" S 64° 8' 19" E
1280422	2001-09-06 02:29	0° 1' 13" N 67° 1' 22" E
1280563	2001-09-12 08:19	11° 23' 39" N 66° 59' 47" E
1280599	2001-09-14 07:47	15° 11' 54" N 66° 59' 56" E
1655122	2001-09-05 08:41	0° 0' 14" S 67° 0' 4" E
1655171	2001-09-07 20:30	3° 47' 50" N 67° 0' 0" E
2273054	2001-09-05 00:26	0° 0' 9" S 66° 59' 55" E
1655379	2001-09-22 08:32	23° 33' 11" N 59° 53' 50" E
2272978	2001-09-20 00:29	20° 54' 48" N 63° 39' 54" E
2273005	2001-09-23 00:31	23° 33' 16" N 59° 54' 1" E
2273171	2001-09-03 01:33	0° 54' 41" S 64° 8' 30" E
2273195	2001-09-05 00:26	0° 0' 9" S 66° 59' 55" E
2273275	2001-09-08 02:24:30	3° 47' 58" N 67° 0' 3" E
2273435	2001-09-17 02:20:30	19° 0' 4" N 67° 0' 0" E
2273540	2001-09-25 06:39:30	24° 18' 45" N 58° 10' 41" E
2273564	2001-09-27 00:24	26° 0' 7" N 56° 35' 3" E
2273588	2001-09-03 04:25:30	0° 54' 15" S 64° 8' 8" E
2273607	2001-09-03 09:01:30	0° 54' 53" S 64° 8' 21" E
1280410	2001-09-05 06:03	0° 1' 13" S 66° 59' 30" E
1280434	2001-09-07 08:41	3° 48' 1" N 67° 0' 2" E
1280631	2001-09-16 14:30	19° 0' 3" N 67° 0' 2" E
1280723	2001-09-24 08:30	24° 19' 55" N 58° 10' 3" E
1280735	2001-09-25 00:31	24° 19' 55" N 58° 10' 3" E
1655251	2001-09-13 00:28	11° 23' 44" N 66° 59' 50" E
1655263	2001-09-14 07:47	15° 11' 54" N 66° 59' 56" E
1655306	2001-09-16 20:32	19° 0' 1" N 67° 0' 11" E
1655411	2001-09-25 00:31	24° 19' 55" N 58° 10' 3" E
2272794	2001-09-06 02:28:30	0° 1' 13" N 67° 1' 22" E
2272917	2001-09-16 08:30:30	18° 59' 58" N 67° 0' 6" E
2272991	2001-09-22 08:32	23° 33' 11" N 59° 53' 50" E
2273030	2001-09-26 08:28	26° 0' 5" N 56° 34' 56" E
2273109	2001-09-15 00:28:30	15° 11' 56" N 67° 0' 1" E
2273183	2001-09-04 03:30:30	0° 54' 40" S 64° 8' 19" E
2273202	2001-09-05 06:02:30	0° 1' 13" S 66° 59' 30" E
2273379	2001-09-14 07:46:30	15° 11' 54" N 66° 59' 56" E
2273644	2001-09-05 08:41	0° 0' 14" S 67° 0' 4" E
2273656	2001-09-06 02:28:30	0° 1' 13" N 67° 1' 22" E
2273693	2001-09-11 00:26	7° 36' 6" N 67° 0' 16" E
2273804	2001-09-24 08:29:30	24° 19' 55" N 58° 10' 3" E
1280378	2001-09-03 07:02	0° 53' 24" S 64° 8' 12" E
1280502	2001-09-09 00:30	3° 47' 26" N 67° 0' 1" E
1280526	2001-09-10 08:35	7° 36' 1" N 67° 0' 15" E
1280655	2001-09-17 02:21	19° 0' 4" N 67° 0' 0" E
1280679	2001-09-19 08:28	20° 54' 49" N 63° 39' 56" E
1280680	2001-09-20 00:29	20° 54' 48" N 63° 39' 54" E
1655078	2001-09-03 01:33	0° 54' 41" S 64° 8' 30" E
1655110	2001-09-05 00:26	0° 0' 9" S 66° 59' 55" E
1655134	2001-09-06 02:29	0° 1' 13" N 67° 1' 22" E
1655146	2001-09-07 08:41	3° 48' 1" N 67° 0' 2" E
1655238	2001-09-12 08:19	11° 23' 39" N 66° 59' 47" E
2272757	2001-09-01 10:40	2° 26' 41" S 59° 22' 15" E
2273042	2001-09-03 01:33	0° 54' 41" S 64° 8' 30" E
2273134	2001-09-23 00:31	23° 33' 16" N 59° 54' 1" E
2273263	2001-09-07 20:29:30	3° 47' 50" N 67° 0' 0" E
2273299	2001-09-09 06:31	3° 45' 57" N 66° 59' 7" E
2273460	2001-09-19 08:28	20° 54' 49" N 63° 39' 56" E
2273736	2001-09-15 00:28:30	15° 11' 56" N 67° 0' 1" E
1280471	2001-09-07 20:30	3° 47' 50" N 67° 0' 0" E
1280514	2001-09-09 06:31	3° 45' 57" N 66° 59' 7" E
1280606	2001-09-15 00:29	15° 11' 56" N 67° 0' 1" E
1280692	2001-09-22 08:32	23° 33' 11" N 59° 53' 50" E
1655202	2001-09-09 00:30	3° 47' 26" N 67° 0' 1" E
1655367	2001-09-20 00:29	20° 54' 48" N 63° 39' 54" E
1655287	2001-09-16 08:31	18° 59' 58" N 67° 0' 6" E
1655423	2001-09-26 08:28	26° 0' 5" N 56° 34' 56" E
2272813	2001-09-07 14:33	3° 47' 57" N 67° 0' 5" E
2272825	2001-09-07 20:29:30	3° 47' 50" N 67° 0' 0" E
2272837	2001-09-08 02:24:30	3° 47' 58" N 67° 0' 3" E
2272905	2001-09-15 00:28:30	15° 11' 56" N 67° 0' 1" E
2273091	2001-09-13 00:27:30	11° 23' 44" N 66° 59' 50" E
2273226	2001-09-07 08:41	3° 48' 1" N 67° 0' 2" E
2273251	2001-09-07 17:28:30	3° 47' 57" N 67° 0' 14" E
2273306	2001-09-10 08:34:30	7° 36' 1" N 67° 0' 15" E
2273380	2001-09-15 00:28:30	15° 11' 56" N 67° 0' 1" E
2273423	2001-09-16 20:31:30	19° 0' 1" N 67° 0' 11" E
2273619	2001-09-04 05:22:30	0° 54' 16" S 64° 8' 15" E
2273797	2001-09-23 06:44:30	23° 31' 59" N 59° 53' 36" E
1280409	2001-09-05 00:26	0° 0' 9" S 66° 59' 55" E
1280483	2001-09-08 02:25	3° 47' 58" N 67° 0' 3" E
1655195	2001-09-08 08:28	3° 47' 33" N 67° 0' 4" E
1655214	2001-09-10 08:35	7° 36' 1" N 67° 0' 15" E
1655275	2001-09-15 00:29	15° 11' 56" N 67° 0' 1" E
1655299	2001-09-16 14:30	19° 0' 3" N 67° 0' 2" E
1655355	2001-09-19 08:28	20° 54' 49" N 63° 39' 56" E
1655435	2001-09-27 00:24	26° 0' 7" N 56° 35' 3" E
2272849	2001-09-09 00:29:30	3° 47' 26" N 67° 0' 1" E
2272898	2001-09-14 07:46:30	15° 11' 54" N 66° 59' 56" E
2273110	2001-09-18 00:28:30	18° 59' 51" N 66° 59' 55" E
2273122	2001-09-20 00:29	20° 54' 48" N 63° 39' 54" E
2273214	2001-09-06 02:28:30	0° 1' 13" N 67° 1' 22" E
2273287	2001-09-09 00:29:30	3° 47' 26" N 67° 0' 1" E
2273496	2001-09-22 08:32	23° 33' 11" N 59° 53' 50" E
2273539	2001-09-25 00:31	24° 19' 55" N 58° 10' 3" E
2273552	2001-09-26 08:28	26° 0' 5" N 56° 34' 56" E
2273668	2001-09-08 08:27:30	3° 47' 33" N 67° 0' 4" E
2273700	2001-09-13 00:27:30	11° 23' 44" N 66° 59' 50" E
2273712	2001-09-13 05:33	11° 22' 41" N 66° 59' 39" E
2273724	2001-09-14 07:46:30	15° 11' 54" N 66° 59' 56" E
2273748	2001-09-17 02:20:30	19° 0' 4" N 67° 0' 0" E
2273785	2001-09-20 06:00:30	20° 55' 9" N 63° 38' 51" E
2273816	2001-09-25 04:46:30	24° 19' 29" N 58° 10' 16" E
2273828	2001-09-25 06:39:30	24° 18' 45" N 58° 10' 41" E

Discrete samples

65 CTD frame plus rosette sampler

Transmittance and attenuance of the water column
Bacterial production in the water column
Regenerated production in water bodies
Bacteria taxonomy-related biomass expressed as carbon per unit volume of the water column
Carotenoid and flavenoid pigment concentrations in water bodies
Particulate total and organic nitrogen concentrations in the water column
Nitrite concentration parameters in the water column
Zooplankton taxonomy-related abundance per unit volume of the water column
Dissolved oxygen parameters in the water column
Microzooplankton taxonomy-related biomass expressed as carbon per unit volume of the water column
Phytoplankton taxonomic abundance in water bodies
Phosphate concentration parameters in the water column
Salinity of the water column
Temperature of the water column
Zooplankton taxonomy-related biomass expressed as carbon per unit volume of the water column
Phytoplankton taxonomic biomass in water bodies
Phytoplankton generic biomass in water bodies
Concentration of suspended particulate material in the water column
Bacteria generic abundance in water bodies
Silicate concentration parameters in the water column
Visible waveband radiance and irradiance measurements in the water column
Zooplankton non taxonomy-related abundance per unit volume of the water column
Ammonium and ammonia concentration parameters in water bodies
Bacteria taxonomic abundance in water bodies
Microzooplankton taxonomic abundance in water bodies
Phytoplankton generic abundance in water bodies
Molecular biology parameters
Density of the water column
Zooplankton non taxonomy-related biomass expressed as carbon per unit volume of the water column
Nitrate concentration parameters in the water column
Metadata parameters
Chlorophyll pigment concentrations in water bodies
Dissolved organic carbon uptake and production in the water column
New production in water bodies
Primary production in the water column

7 Fast Repetition Rate Fluorometer

11 In situ productivity incubations

61 Moving Vessel Profiler instrument

Speed of sound and travel time in the water column
Salinity of the water column
Temperature of the water column
Density of the water column
Raw fluorometer output
Chlorophyll pigment concentrations in water bodies

98 Sample taken from ship's non-toxic supply

Salinity of the water column
Temperature of the water column
Chlorophyll pigment concentrations in water bodies

18 Stand-alone pumps

1 Surface water sample collected by a bucket on a rope

Phytoplankton taxonomic abundance in water bodies
Phytoplankton generic abundance in water bodies

12 Unspecified zooplankton net or nets

7 Water bottle hydrocast

1 Water sample pooled from multiple CTD-rosette stations

Resources

RRS Charles Darwin CD132 (AMBITION)

Cruise summary report

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