Cruise inventory

RV Cefas Endeavour 08/11

Cruise summary report

Cruise Info. 
Ship name (ship code)RV Cefas Endeavour (74E9)
Cruise identifier08/11
Cruise period2011-05-08 — 2011-05-13
Port of departureLowestoft, United Kingdom
Port of returnLowestoft, United Kingdom

ProTool Aims
a. To calibrate the variable fluorescence technique against 14C or 13C -based photosynthesis measurements for phytoplankton samples collected from a range of ecohydrodynamic regions
b. To demonstrate the use of an on-line automated flow-through system using the variable fluorescence technique throughout the cruise (not done due to staff illness)
c. To investigate diurnal variability in the phytoplankton photophysiology
d. To measure variability in spectral irradiance of the water column at different locations (not done due to equipment failure)
e. To measure low-temperature fluorescence excitation and emission spectra of phytoplankton throughout the cruise
f. To measure size-fractionated primary production using the variable fluorescence method
g. To map the distribution of "cDOM" type substances which contribute to the background fluorescence of the variable fluorescence fluorimeters
h. To measure the effect of nutrient addition to phytoplankton exhibiting low fluorescence yields (done, at one station)

DyMaphy Aims
a. To compare the analyses of functional groups of phytoplankton with different flow cytometers in different water bodies and in different conditions of nutrients and light (tank experiment)
b. To compare results and performance of a portable Ferrybox with the on board Ferrybox
c. To test the feasibility of analysing functional groups with on-line continuous flow with flow cytometry

UEA Aims

a. (Queste) To deploy and retrieve a Seaglider for the first time in the North Sea
b. (Owen)To estimate biovolume for different functional groups of phytoplankton by comparing carbon, chlorophyll and DNA content with flow cytometric analyses

U Bourne Aims

a. Use an Accuri C6 flow cytometer to quantify the abundance of phytoplankton <15-20 microns in size (Synechococcus, picoeukaryotes and some nanoeukaryotes).
b. Assess the 'viability' of these fractions of the phytoplankton by incubating samples with the cell stains CMFDA and SYTOX-green and to derive an index of physiological condition across different hydrographic conditions.
c. Analyse water samples for the presence of chlorophyll, and the chlorophyll alteration products methoxychlorophyll a and hydroxychlorophyll a by HPLC as indicators of chlorophyll turnover and cell senescence.(done)

Secondary Aims

a. Continued calibration and utilisation of FerryBox on Cefas Endeavour.
Chief scientistRodney Forster (Centre for Environment, Fisheries and Aquaculture Science Lowestoft Laboratory)
ProjectProTool1, DyMaphy
Cruise reportRV Cefas Endeavour 08/11 cruise report endeavour08_11.pdf — access key 'C' (0.08 MB) 
Ocean/sea areas 
GeneralNorth Sea
SpecificSouthern and central North Sea
Track chartsRV Cefas Endeavour 08/11 cruise track — endeavour08_11trk.pdf (0.04 MB)