RV Cefas Endeavour 08/11
Cruise summary report
| Cruise Info. | |
| Ship name (ship code) | RV Cefas Endeavour (74E9) |
| Cruise identifier | 08/11 |
| Cruise period | 2011-05-08 — 2011-05-13 |
| Status | Completed |
| Port of departure | Lowestoft, United Kingdom |
| Port of return | Lowestoft, United Kingdom |
| Purpose | Research |
| Objectives | Aims
ProTool Aims a. To calibrate the variable fluorescence technique against 14C or 13C -based photosynthesis measurements for phytoplankton samples collected from a range of ecohydrodynamic regions b. To demonstrate the use of an on-line automated flow-through system using the variable fluorescence technique throughout the cruise (not done due to staff illness) c. To investigate diurnal variability in the phytoplankton photophysiology d. To measure variability in spectral irradiance of the water column at different locations (not done due to equipment failure) e. To measure low-temperature fluorescence excitation and emission spectra of phytoplankton throughout the cruise f. To measure size-fractionated primary production using the variable fluorescence method g. To map the distribution of "cDOM" type substances which contribute to the background fluorescence of the variable fluorescence fluorimeters h. To measure the effect of nutrient addition to phytoplankton exhibiting low fluorescence yields (done, at one station) DyMaphy Aims a. To compare the analyses of functional groups of phytoplankton with different flow cytometers in different water bodies and in different conditions of nutrients and light (tank experiment) b. To compare results and performance of a portable Ferrybox with the on board Ferrybox c. To test the feasibility of analysing functional groups with on-line continuous flow with flow cytometry UEA Aims a. (Queste) To deploy and retrieve a Seaglider for the first time in the North Sea b. (Owen)To estimate biovolume for different functional groups of phytoplankton by comparing carbon, chlorophyll and DNA content with flow cytometric analyses U Bourne Aims a. Use an Accuri C6 flow cytometer to quantify the abundance of phytoplankton <15-20 microns in size (Synechococcus, picoeukaryotes and some nanoeukaryotes). b. Assess the 'viability' of these fractions of the phytoplankton by incubating samples with the cell stains CMFDA and SYTOX-green and to derive an index of physiological condition across different hydrographic conditions. c. Analyse water samples for the presence of chlorophyll, and the chlorophyll alteration products methoxychlorophyll a and hydroxychlorophyll a by HPLC as indicators of chlorophyll turnover and cell senescence.(done) Secondary Aims a. Continued calibration and utilisation of FerryBox on Cefas Endeavour. |
| Chief scientist | Rodney Forster (Centre for Environment, Fisheries and Aquaculture Science Lowestoft Laboratory) |
| Project | ProTool1, DyMaphy |
| Cruise report |  (0.08 MB) |
| Ocean/sea areas | |
| General | North Sea |
| Specific | Southern and central North Sea |
| Track charts | (0.04 MB) |
| Measurements | |
| Physical oceanography | |
| Surface measurements underway (T,S) | Quantity: activity duration in days = 6 Description: 2 Ferrybox systems (Cefas and Ifremer) |
| Water bottle stations | Quantity: number of samples = 17 Description: Water bottle stations |
| CTD stations | Quantity: number of profiles = 19 Description: CTD-Stations |
| Subsurface temperature and salinity measurements | Quantity: activity duration in hours = 10 Description: Glider deployment |
| Transparency (eg transmissometer) | Quantity: activity duration in hours = 10 Description: Glider deployment |
| Optics (eg underwater light levels) | Quantity: number of profiles = 1 Description: attached to CTD |
| Chemical oceanography | |
| Oxygen | Quantity: activity duration in days = 6 Description: 2 Ferrybox systems (Cefas and Ifremer) |
| Oxygen | Quantity: activity duration in hours = 10 Description: Glider deployment |
| Biology and fisheries | |
| Primary productivity | Quantity: number of samples = 17 Description: Primary productivity |
| Phytoplankton pigs (eg chlorophyll, fluorescence) | Quantity: activity duration in days = 6 Description: 2 Ferrybox systems (Cefas and Ifremer) |
| Phytoplankton pigs (eg chlorophyll, fluorescence) | Quantity: number of stations = 17 Description: Phytoplankton pigments (e.g. chlorophyll, fluorescence) |
| Phytoplankton pigs (eg chlorophyll, fluorescence) | Quantity: activity duration in hours = 10 Description: Glider deployment |
| Dissolved organic matter (inc DOC) | Quantity: number of stations = 17 Description: Dissolved organic matter (e.g. DOC) |
| Phytoplankton | Quantity: number of samples = 17 Description: Phytoplankton |
