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RRS Charles Darwin CD132 (AMBITION)

Cruise summary report

Cruise Info. 
Ship name (ship code)RRS Charles Darwin (74AB)
Cruise identifierCD132 (AMBITION)
Cruise period2001-08-30 — 2001-09-29
StatusCompleted
Port of departureVictoria, Seychelles
Port of returnMuscat, Oman
PurposeResearch
ObjectivesThe objectives of the five participating M&FMB projects were:
1) To collect material (preserved filtrates and frozen samples) for DNA extraction and analysis on return to Cardiff, particularly in more eutrophic waters [Fry & Weightman (Cardiff): Isolation and characterisation of unculturable? Heterotrophic marine bacteria].
2) To sample the upper-ocean for methyl bromide (MeBr) and isolation of MeBr-utilising bacteria. Carry out onboard experiments using gas chromatographs, liquid nitrogen, gas bottles and incubators. [Murrell & McDonald (Warwick), Nightingale (PML): Methyl bromide metabolism in the marine environment ]
3) To sample the horizontal and vertical distributions of picoeukaryotes in relation to environmental gradients. Also investigate temporal responses (diel changes in situ and via deck incubations) and carry out DNA and RNA analyses. [Scanlan (Warwick): In situ community structure of marine photosynthetic picoeukaryotes ]
4) To generate a range of size-fractionated samples for analysis of nif gene diversity, together with information on environmental conditions (to determine factors affecting gene expression). [Wyman (Stirling) & O?Donnell (Newcastle): Nitrogen fixing microbes in the sea - a molecular approach linking phenotype to 16S rRNA phylotype]
5) To characterise bacterioplankton community and to investigate their links to biogeochemical functions. [Zubkov & Burkill (PML): Bacterial biodiversity; dominance, competitiveness and functionality within communities]
Chief scientistPeter H Burkill (Plymouth Marine Laboratory)
ProjectMarine and Freshwater Microbial Biodiversity (M&FMB)
Cruise reportRRS Charles Darwin CD132 (AMBITION) cruise report cd132.pdf (3.72 MB) 
Ocean/sea areas 
GeneralIndian Ocean
Arabian Sea
Track chartsRRS Charles Darwin CD132 (AMBITION) cruise track — cd132trk.pdf (0.47 MB) 
Measurements 
Physical oceanography 
CTD stationsDescription: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples
Biology and fisheries 
Phytoplankton pigs (eg chlorophyll, fluorescence)Description: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples
PhytoplanktonDescription: Lots of samples were taken throughout the cruise. FRRF deployments Nucleic acid analysis samples Microplankton Phytoplankton Bacterioplankton Nitrogen flux experiments CTD samples
Data held at BODC Get data
Discrete samples 
65  CTD frame plus rosette samplerBacterial production in the water column
Transmittance and attenuance of the water column
Regenerated production in water bodies
Bacteria taxonomy-related biomass expressed as carbon per unit volume of the water column
Carotenoid and flavenoid pigment concentrations in water bodies
Particulate total and organic nitrogen concentrations in the water column
Nitrite concentration parameters in the water column
Temperature of the water column
Zooplankton taxonomy-related abundance per unit volume of the water column
Dissolved oxygen parameters in the water column
Microzooplankton taxonomy-related biomass expressed as carbon per unit volume of the water column
Phytoplankton taxonomic abundance in water bodies
Phosphate concentration parameters in the water column
Salinity of the water column
Concentration of suspended particulate material in the water column
Zooplankton taxonomy-related biomass expressed as carbon per unit volume of the water column
Phytoplankton taxonomic biomass in water bodies
Phytoplankton generic biomass in water bodies
Silicate concentration parameters in the water column
Visible waveband radiance and irradiance measurements in the water column
Bacteria generic abundance in water bodies
Zooplankton non taxonomy-related abundance per unit volume of the water column
Molecular biology parameters
Density of the water column
Ammonium and ammonia concentration parameters in water bodies
Bacteria taxonomic abundance in water bodies
Microzooplankton taxonomic abundance in water bodies
Phytoplankton generic abundance in water bodies
Metadata parameters
Zooplankton non taxonomy-related biomass expressed as carbon per unit volume of the water column
Nitrate concentration parameters in the water column
Dissolved organic carbon uptake and production in the water column
Chlorophyll pigment concentrations in water bodies
New production in water bodies
Primary production in the water column
7  Fast Repetition Rate Fluorometer
11  In situ productivity incubations
61  Moving Vessel Profiler instrumentSound velocity and travel time in the water column
Temperature of the water column
Salinity of the water column
Density of the water column
Raw fluorometer output
Chlorophyll pigment concentrations in water bodies
98  Sample taken from ship's non-toxic supplyTemperature of the water column
Salinity of the water column
Chlorophyll pigment concentrations in water bodies
18  Stand-alone pumps
1  Surface water sample collected by a bucket on a ropePhytoplankton taxonomic abundance in water bodies
Phytoplankton generic abundance in water bodies
12  Unspecified zooplankton net or nets
7  Water bottle hydrocast
1  Water sample pooled from multiple CTD-rosette stations