Metadata Report for BODC Series Reference Number 2246437

• Metadata Summary

• Problem Reports

• Data Access Policy

• Narrative Documents

• Project Information

• Data Activity or Cruise Information

• Fixed Station Information

• BODC Quality Flags

• SeaDataNet Quality Flags

Metadata Summary

Problem Reports

No Problem Report Found in the Database

Data Access Policy

Open Data

These data have no specific confidentiality restrictions for users. However, users must acknowledge data sources as it is not ethical to publish data without proper attribution. Any publication or other output resulting from usage of the data should include an acknowledgment.

If the Information Provider does not provide a specific attribution statement, or if you are using Information from several Information Providers and multiple attributions are not practical in your product or application, you may consider using the following:

"Contains public sector information licensed under the Open Government Licence v1.0."

Narrative Documents

Niskin Bottle

The Niskin bottle is a device used by oceanographers to collect subsurface seawater samples. It is a plastic bottle with caps and rubber seals at each end and is deployed with the caps held open, allowing free-flushing of the bottle as it moves through the water column.

Standard Niskin

The standard version of the bottle includes a plastic-coated metal spring or elastic cord running through the interior of the bottle that joins the two caps, and the caps are held open against the spring by plastic lanyards. When the bottle reaches the desired depth the lanyards are released by a pressure-actuated switch, command signal or messenger weight and the caps are forced shut and sealed, trapping the seawater sample.

Lever Action Niskin

The Lever Action Niskin Bottle differs from the standard version, in that the caps are held open during deployment by externally mounted stainless steel springs rather than an internal spring or cord. Lever Action Niskins are recommended for applications where a completely clear sample chamber is critical or for use in deep cold water.

Clean Sampling

A modified version of the standard Niskin bottle has been developed for clean sampling. This is teflon-coated and uses a latex cord to close the caps rather than a metal spring. The clean version of the Levered Action Niskin bottle is also teflon-coated and uses epoxy covered springs in place of the stainless steel springs. These bottles are specifically designed to minimise metal contamination when sampling trace metals.

Deployment

Bottles may be deployed singly clamped to a wire or in groups of up to 48 on a rosette. Standard bottles and Lever Action bottles have a capacity between 1.7 and 30 L. Reversing thermometers may be attached to a spring-loaded disk that rotates through 180° on bottle closure.

Total Bacterioplankton and Phytoplankton measurements from CTD bottle samples taken during CROZEX cruises D285 and D286

Originator's Protocol for Data Acquisition and Analysis

Water samples for the determination of total bacterioplankton and phytoplankton concentrations were drawn from Niskin bottles from a 24-rosette sampling system mounted on a Sea-Bird 9/11 plus CTD. Sixteen of the sampled casts were carried using a stainless steel CTD and one (station 15496) from a titanium CTD.

Seawater samples were collected in acid washed 50 mL polypropylene tubes from a CTD system. Samples were stored in a refrigerator and microorganisms were preserved with paraformaldehyde (1% final concentration) within 1-2 hours of collection. Phytoplankton samples were analysed unstained and bacterioplankton samples were stained with SYBR Green I nucleic acid dye. The bacterial samples were then left in the dark at 35^oC for at least 1 hour before enumeration of bacterioplankton by a Becton Dickinson FACSort flow cytometer. Total numbers of bacterioplankton and phytoplankton cells were recorded, as well as total numbers of individuals within different bacterioplankton separated by DNA content populations which have been loaded elsewhere.

After station 15496, it was decided that samples should not be taken from depths greater than 200 m, owing to low phytoplankton and bacterioplankton abundance at greater depths. Alterations in bottle firing sequence however, denoted that some stations had to be sampled to a depth of 250 m, in order to obtain data for depths greater than 175 m. No data were recorded for bacterioplankton at station 15496 due to an error in the preservation/SYBR Green staining procedure.

For further information see the cruise report as well as Marie et al. (1997) and Zubkov et al. (2001).

References Cited

Zubkov, M.V., Fuchs, B.M., Burkill, P.H., Aman, R. 2001. Comparison of Cell Specific Activities of Dominant Bacterioplankton Groups in Stratified Waters of the Celtic Sea. Applied Environmental Microbiology, 67, 5210-5218.

Marie, D., Partensky, F., Jacquet, S., and Vaulot, D., 1997. Enumeration and cell cycle analysis of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green. Applied Environmental Microbiology, 63, 186-193.

BODC processing

All data were taken from the cruise report pages 174 to 178 and were loaded into the BODC database using established BODC data banking procedures. Data were screened in-house prior to loading. The following table shows how the variables were mapped to appropriate BODC parameter codes:

Data Quality Report

None (BODC assessment).

Problem Report

None (BODC assessment).

Bacterioplankton and Phytoplankton species measurements from CTD bottle samples taken during CROZEX cruises D285 and D286

Originator's Protocol for Data Acquisition and Analysis

Water samples for the determination of bacterioplankton and phytoplankton were drawn from 20 litre Niskin bottles from a 24-rosette sampling system mounted on a stainless steel Sea-Bird 9/11 plus CTD.

Seawater samples were collected in acid washed 50 mL polypropylene tubes from a CTD system. Samples were stored in a refrigerator and microorganisms were preserved with paraformaldehyde (1% final concentration) within 1-2 hours of collection. Phytoplankton samples were analysed unstained and bacterioplankton samples were stained with SYBR Green I nucleic acid dye. The bacterial samples were then left in the dark at 35^oC for at least 1 hour before enumeration of bacterioplankton by a Becton Dickinson FACSort flow cytometer. Total numbers of individuals within different bacterioplankton separated by DNA content populations were recorded as well as total numbers of bacterioplankton and phytoplankton cells which were loaded elsewhere.

After station 15496, it was decided that samples should not be taken from depths greater than 200 m, owing to low phytoplankton and bacterioplankton abundance at greater depths. Alterations in bottle firing sequence however, denoted that some stations had to be sampled to a depth of 250 m, in order to obtain data for depths greater than 175 m. No data were recorded for bacterioplankton at station 15496 due to an error in the preservation/SYBR Green staining procedure.

For further information see the cruise report as well as Marie et al. (1997) and Zubkov et al. (2001).

References Cited

Zubkov, M.V., Fuchs, B.M., Burkill, P.H., Aman, R. 2001. Comparison of Cell Specific Activities of Dominant Bacterioplankton Groups in Stratified Waters of the Celtic Sea. Applied Environmental Microbiology, 67, 5210-5218.

Marie, D., Partensky, F., Jacquet, S., and Vaulot, D., 1997. Enumeration and cell cycle analysis of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green. Applied Environmental Microbiology, 63, 186-193.

BODC processing

All data were received in one Microsoft Excel format file and were loaded into the BODC database using established BODC data banking procedures. Data were screened in-house prior to loading. The following table shows how the variables were mapped to appropriate BODC parameter codes:

Data Quality Report

None (BODC assessment).

Problem Report

None (BODC assessment).

Project Information

CROZet natural iron bloom EXport experiment (CROZEX)

The multidisciplinary CROZet natural iron bloom EXport experiment (CROZEX) was a major component of the Natural Environment Research Council (NERC) funded core strategic project Biophysical Interactions and Controls over Export Production (BICEP). The project is the first planned natural iron fertilisation experiment to have been conducted in the Southern Ocean.

The overall objective of CROZEX was to examine, from surface to sediment, the structure, causes and consequences of a naturally occurring phytoplankton bloom in the Southern Ocean. The Crozet Plateau was chosen as the study area. This area typically exhibits two phytoplankton blooms a year, a primary bloom in that peaks in October and a secondary bloom in December or January. Specific aims with respect to these were to:

• Determine what limits the primary bloom
• Determine the cause of the secondary bloom

The project was run by the George Deacon Division (GDD), now Ocean Biogeochemistry and Ecosystems (OBE) at the National Oceanography Centre Southampton (NOCS). Participants from five other university departments also contributed to the project.

The project ran from November 2004 to January 2008 with marine data collection between 3^rd November 2004 and 21^st January 2005. There were 2 cruises to the Crozet Islands Plateau, which are summarised in Table 1.

Table 1: Details of the RRS Discovery CROZEX cruises.

The two cruises aimed to survey two areas at different phases of the bloom cycle described above. A control area to the south of the Crozet Islands, which is classified as High Nutrient Low Chorophyll (HNLC), where the blooms do not occur and a second area in the region of the blooms to the north of the Crozet Islands.

Sampling was undertaken at ten major stations (see Pollard et al., 2007) numbered M1 to M10. The following observations/sampling were conducted at each station where possible:

• Several CTD casts sampling:
  • Iron (using a titanium rig)
  • ²³⁴Th
  • Physical parameters (temperature, salinity etc)
  • Oxygen
  • CO₂
  • Nutrients using a stainless steel rig including a Lowered Acoustic Doppler Current Profiller (LADCP)
• At each thorium cast there was an associated Stand Alone Pump System (SAPS) deployment
• At some stations, a drifting PELAGRA trap was deployed for the duration of the work
• Megacoring was undertaken at M5 and M6
• Gravity coring was undertaken at M5, M6 and M10
• Longhurst Hardy Plankton Recorder (LPHR) tows were undertaken at a few major stations

For each of the major stations (M1 to M10), the following were determined:

• Primary productivity
• New Production
• Phytoplankton community composition
• Bacterial activity
• Iron
• Nutrient drawdown
• Thorium export

Sampling between major stations included:

• SeaSoar runs instrumented with:
  • CTD
  • Optical Plankton Counter (OPC)
  • Fast Repetition Rate fluorimeter (FRRf)
• Physics CTD casts on several lines
• Argo float deployments
• Zooplankton nets at nearly every CTD and major station
• Underway and on-station CO₂ measurements
• Underway nutrients and radium sampling
• 5 to 6 day ship-board iron-addition incubation experiments
• Checks against near-real-time satellite and model data
• Mooring deployments based on the satellite imagery in support of the CROZET (Benthic CROZEX) project.

The CROZEX cruises included 6 extra days in support of the CROZET (Benthic CROZEX) project, whose main cruise took place one year after the CROZEX cruises. The CROZET work undertaken during the CROZEX cruises was primarily the moored sediment trap deployments, although some of the coring work is applicable to both projects.

CROZEX produced significant findings in several disciplines, including confirmation that iron from Crozet fertilised the bloom and that phytoplankton production rates and most export flux estimates were much larger in the bloom area than the HNLC area (Pollard et al. 2007). Many of the project results are presented in a special CROZEX issue of Deep-Sea Research II (volume 54, 2007).

References

Pollard R., Sanders R., Lucas M. and Statham P., 2007. The Crozet natural iron bloom and export experiment (CROZEX). Deep-Sea Research II, 54, 1905-1914.

Data Activity or Cruise Information

Data Activity

BODC Sample Metadata Report for D285_CTD_15520

Please note:the supplied parameters may not have been sampled from all the bottle firings described in the table above. Cross-match the Sample Reference Number above against the SAMPRFNM value in the data file to identify the relevant metadata.

Related Data Activity activities are detailed in Appendix 1

Cruise

Complete Cruise Metadata Report is available here

Fixed Station Information

No Fixed Station Information held for the Series

BODC Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

SeaDataNet Quality Control Flags

The following single character qualifying flags may be associated with one or more individual parameters with a data cycle:

Appendix 1: D285_CTD_15520

Related series for this Data Activity are presented in the table below. Further information can be found by following the appropriate links.

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