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RV Kilo Moana KM1128 (GPc03, METZYME)

Cruise plan

Cruise Info. 
Ship name (ship code)RV Kilo Moana (33KB)
Cruise identifierKM1128 (GPc03, METZYME)
Cruise period2011-10-01 — 2011-10-25
StatusCompleted
Port of departureHonolulu, United States
Port of returnApia, Samoa
PurposeResearch
ObjectivesMetZyme project researchers will determine the role of enzymatic activity in the cycling of trace metals. Specifically the research will address the following questions: (1) degradation of sinking particulate organic material in the Tropical North Pacific can be influenced by the ability of microbes to synthesize zinc proteases, which in turn is controlled by the abundance or availability of zinc, and (2) methylation of mercury is controlled, in part, by the activity of cobalt-containing enzymes, and therefore the supply of labile cobalt to the corrinoid-containing enzymes or co-factors responsible for methylation. To attain their goal, they will collect dissolved and particulate samples for trace metals and metalloenzymes from three stations along a biogeochemical gradient in the Tropical North Pacific (along 150 degrees West from 18 degrees North to the equator). Sinking particles from metal clean sediment traps will also be obtained. The samples will also be used to carry out shipboard incubation experiments using amendments of metals, metal-chelators, B12, and proteases to examine the sensitivity and metal limitation of heterotrophic, enzymatic degradation of organic matter within the oceanic "Twilight Zone" (100-500 m). This study will result in a novel metaproteomic/metalloenzyme datasets that should provide insights into the biogeochemical cycling of metals, as well as co-limitation of primary productivity and controls on the export of carbon from the photic zone. In addition to the final data being contributed to BCO-DMO, an online metaproteomic data server will be created so the community has access to the raw data files generated by this research.
Chief scientistCarl H Lamborg (Woods Hole Oceanographic Institution Department of Marine Chemistry and Geochemistry)
Ocean/sea areas 
GeneralNorth East Pacific Ocean (limit 180W)
SpecificEquatorial and Central Pacific, Tongan Plateau
Data held at BODC 
Discrete samples 
8  CTD frame plus rosette samplerCarotenoid and flavenoid pigment concentrations in water bodies
Chlorophyll pigment concentrations in water bodies
11  Stand-alone pumpsConcentration of proteins in the water column
31  Nitrite concentration parameters in the water column
Carotenoid and flavenoid pigment concentrations in water bodies
Temperature of the water column
Phosphate concentration parameters in the water column
Salinity of the water column
Dissolved oxygen parameters in the water column
Silicate concentration parameters in the water column
Ammonium and ammonia concentration parameters in water bodies
Nitrate concentration parameters in the water column
Dissolved metal concentrations in the water column
Chlorophyll pigment concentrations in water bodies